Biotechnology Letters

, Volume 18, Issue 10, pp 1145–1148 | Cite as

Expression parameters for target gene cloned in Escherichia coli in response to phosphate supply

  • Sanjoy K. Bhattacharya
  • Ashok K. Dubey
Article

Summary

Recombinant strain of E.coli 1727 overexpressed target gene at enhanced level when supplied with excess of inorganic phosphate. Rate of target gene expression, yield coefficient for target gene product and plasmid copy number increased significantly: 50%, 100% and 40% respectively at 125 mM excess phosphate. This was, however, accompanied by 26% decrease in specific growth rate and 30% in cellular growth yield coefficient.

Keywords

Phosphate Target Gene Gene Product Specific Growth Specific Growth Rate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Sanjoy K. Bhattacharya
    • 1
  • Ashok K. Dubey
    • 1
  1. 1.Department of Biochemical Engineering and BiotechnologyIndian Institute of Technology-DelhiNew DelhiIndia

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