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Biotechnology Letters

, Volume 15, Issue 3, pp 307–310 | Cite as

Stimulation of solavetivone synthesis in free and immobilized cells of Hyoscyamus muticus by Rhizoctonia solani fungal components

  • S. V. Ramakrishna
  • G. Ramakrishna Reddy
  • Wayne R. Curtis
  • Arthur E. Humphrey
Article

Summary

Three elicitor fractions including spent culture medium, hydrolyzed mycelial pellet, and hydrolyzed mycelial biomass supernatant were tested for their ability to induce sesquiterpene formation in freely suspended and immobilized cells. Immobilization enhanced the effectiveness of the hydrolysate elicitors, but not that derived from spent culture medium. When effectiveness of the elicitors is compared based on product yield, spent culture medium is shown to be far more effective than hydrolysates as an elicitor.

Keywords

Biomass Organic Chemistry Immobilization Product Yield Immobilize Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Asada, M. and Shuler, M.L. (1989). Appl. Microbiol. Biotechnol. 30, 475–481.Google Scholar
  2. DiCosmo, F., Quesnel, A., Misawa, M., and Tallevi, S.G. (1987). Appl. Biochem. Biotechnol. 14, 101–106.Google Scholar
  3. Dixon, R.A. and Lamb, C.J. (1979). Biochim. Biophys. Acta. 586, 453–463.Google Scholar
  4. Dunlop, D.S. and Curtis, W.R. (1991). Biotechnol. Prog. 7, 434–438.Google Scholar
  5. Eilert, U., Constabel, F., and Kurz, G.W. (1986). J. Plant Physiol. 126, 11–22.Google Scholar
  6. Keen, N.T., Partridge, J.E., and Zaki, A.I. (1972). Phytopathology 62, 768.Google Scholar
  7. Malpathak, N.P. and David, S.B. (1992). Biotechnol. Lett. 14(10), 965–968.Google Scholar
  8. Rokem, J.S., Schwarzberg, J., and Goldberg, I. (1984). Plant Cell Rep. 3, 159–160.Google Scholar
  9. Sharp, J.K., McNeil, M., and Albersheim, P. (1984). J. Biol. Chem. 259(18), 11321–11336.Google Scholar
  10. Signs, M. and Flores, H.E. (1989). Plant Phys. (Suppl) 4, 135.Google Scholar

Copyright information

© Science and Technology Letters 1993

Authors and Affiliations

  • S. V. Ramakrishna
    • 1
  • G. Ramakrishna Reddy
    • 1
  • Wayne R. Curtis
    • 1
  • Arthur E. Humphrey
    • 1
  1. 1.Biotechnology Institute, Wartik Laboratory, Pennsylvania State UniversityUniversity ParkUSA

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