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Biotechnology Letters

, Volume 15, Issue 3, pp 301–306 | Cite as

Production of solavetivone by immobilized cells of Hyoscyamus muticus

  • S. V. Ramakrishna
  • G. Ramakrishna Reddy
  • Wayne R. Curtis
  • Arthur E. Humphrey
Article

Summary

The secretion of solavetivone, a phytoalexin, by the cells of Hyoscyamus muticus in response to fungal elicitation has been enhanced by gel entrapment in calcium alginate. The immobilized cells produced 53% higher product and exhibited sustained biosynthetic activity in repeated batch cycles when compared with suspended cells. Providing a non-elicited media exchange and a sufficient interval of time between repeated infection (elicitation) gave greater productivity. Apparently the cells need a period to recover from the infection.

Keywords

Calcium Organic Chemistry Suspended Cell Alginate Media Exchange 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Science and Technology Letters 1993

Authors and Affiliations

  • S. V. Ramakrishna
    • 1
  • G. Ramakrishna Reddy
    • 1
  • Wayne R. Curtis
    • 1
  • Arthur E. Humphrey
    • 1
  1. 1.Biotechnology Institute, Wartik Laboratory, Pennsylvania State UniversityUniversity ParkUSA

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