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Biotechnology Letters

, Volume 15, Issue 3, pp 257–262 | Cite as

Characterization of oat lipase for lipolysis of rice bran oil

  • Jaspreet Kaur
  • V. Ramamurthy
  • R. M. Kothari
Article

Summary

Lipases from plant and microbial sources were screened for lipolysis of rice bran oil (RBO). Oat lipase was found to give reproducibly 60% lipolysis. Factors affecting the efficiency of lipolysis such as oil:emulsifier ratio, duration of homogenization for the substrate preparation, substrate:enzyme ratio, substrate pH, kinetics of lipolysis and efficiency of lipase as a function of pH, oat particle size, its quality and reuseability were studied to assess the feasibility of using of oat lipase for splitting RBO commercially. The efficiency of lipolysis was optimal at (a) RBO:emulsifier (0.5:1) ratio, homogenized for 2.5 min, (b) pH 7.5 and (c) substrate:enzyme ratio of 5 ml: 2 g.

Keywords

Enzyme Particle Size Organic Chemistry Lipase Bran 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Science and Technology Letters 1993

Authors and Affiliations

  • Jaspreet Kaur
    • 1
    • 2
  • V. Ramamurthy
    • 1
    • 2
  • R. M. Kothari
    • 1
    • 2
  1. 1.Biotechnology DivisionThapar Corporate R&D CentrePatialaIndia
  2. 2.School of Biotechnology, Thapar Institute of Engineering & TechnologyPatialaIndia

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