Optimization of fermentation conditions for ethanol production from whey
Summary
Optimal conditions for ethanol production in 7% whey solutions by the yeast Candida pseudotropicalis ATCC 8619 included initial pH of 4.57 and 30°C. Complete fermentation of the available lactose took place without supplementary nutrients; additions of nitrogen or phosphorus salts, yeast extract or corn steep liquor resulted in increased yeast production and lower ethanol yields. A positive correlation was observed between increases in yeast inocula and lactose utilization and ethanol production rates; 8.35 g/l of ethanol was obtained within 22 h by using yeast inoculum of 13.9 g/l. No differences in fermentation rates or ethanol yields were observed when whole or deproteinized whey solutions were used. Concentrated whey permeates, obtained after removal of the valuable proteins from whey, can be effectively fermented for ethanol production.
Keywords
Fermentation Lactose Candida Ethanol Production Ethanol YieldPreview
Unable to display preview. Download preview PDF.
References
- Brown,S.W., and Oliver,S.G. (1982). Biotechnol. Letterrs. 4, 269–274.Google Scholar
- Castillo,F.J., and Sanchez,S.B. (1978). Acta Cient.Venez. 29, 113–118.Google Scholar
- Chen,S.L. (1981). Biotechnol.Bioeng. 23, 1827–1836.Google Scholar
- Cysewski,G.R., and Wilke,C.R. (1977). Biotechnol. Bioeng. 19, 1125–1143.Google Scholar
- Del Rosario,E.J.,Lee,K.J.,and Rogers,P.L. (1979). Biotechnol.Bioeng. 21, 1477–1482.Google Scholar
- Demott,B.J., Draughon,F.A., and Herald,P.J. (1981). J.Food Protection. 44, 588–590.Google Scholar
- Haraldson,Å.,and Björling,T. (1981). Eur.J.Appl.Microbiol.Biotechnol. 13, 34–38.Google Scholar
- Harrison,J.S.,and Graham,J.C.J. (1970). Yeasts in distillery practice. In. The Yeasts, A.H.Rose and J.S.Harrison, eds. vol.3, pp.283–348. London: Academic Press.Google Scholar
- Izaguirre,M.E.,and Castillo,F.J. (1982). Biotechnol. Letters. 4, 257–262.Google Scholar
- Kosikowski,F.V.,and Wzorek,W. (1977). J.Dairy Sci. 60, 1982–1986.Google Scholar
- Krouwel,P.G.,and Braber,L. (1979). Biotechnol. Letters. 1, 403–408.Google Scholar
- Lee,J.H., Williamson,D., and Rogers,P.L. (1980). Biotechnol. Letters. 2, 141–146.Google Scholar
- Lee,K.J., Skotnicki,M.L., Tribe,D.E., and Rogers,P.L. (1981). Biotechnol. Letters. 3, 291–296.Google Scholar
- Lyness,E., and Doelle,H.W. (1980). Biotechnol.Letters. 2, 549–554.Google Scholar
- Maddox,I.S. (1980). Biotechnol. Letters. 2, 493–498.Google Scholar
- Moulin,G.,and Galzy,P. (1981). Alcohol production from whey. In. Advances in Bio-technology, M.Moo-Young and C.W.Robinson, eds, vol.2, pp.181–187. Toronto: Pergamon Press.Google Scholar
- Moulin,G., Laham-Guillaume,M., and Galzy,P. (1980). Biotechnol.Bioeng. 22, 1277–1281.Google Scholar
- Nagodawithana,T.W.,Castellano,C., and Steinkraus,K.H. (1974). Appl.Microbiol. 28, 383–391.Google Scholar
- Navarro,J.M.,and Durand,G. (1978). Ann.Microbiol. 1298, 215–224.Google Scholar
- Rogosa,M., Browne,H.H.,and Whittier,E.O. (1947). J.Dairy.Sci. 30, 263–269.Google Scholar
- Yoo,B.W. (1975). Disser.Abstr.Int. 36, 641B.Google Scholar