Biotechnology Letters

, Volume 17, Issue 9, pp 893–898 | Cite as

Isolation of a highly enantioselective epoxide hydrolase from Rhodococcus sp. NCIMB 11216

  • Martin Mischitz
  • Kurt Faber
  • Andrew Willetts
Article

Summary

Whole cells of Rhodococcus sp. NCIMB 11216 catalyze the asymmetric hydrolysis of racemic epoxides giving access to chiral epoxides and diols, which are important chiral building blocks for the synthesis of bioactive compounds. Employing a four-step purification procedure, the epoxide hydrolase responsible for the reaction was isolated and characterized to be a cofactor-independent, soluble monomeric protein of ~35kDa, exhibiting an isoelectric point of 4.7.

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Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Martin Mischitz
    • 1
  • Kurt Faber
    • 1
  • Andrew Willetts
    • 2
  1. 1.Institute of Organic Chemistry, Graz University of TechnologyGrazAustria
  2. 2.Department of Biological SciencesExeter UniversityExeterUK

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