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Journal of Muscle Research & Cell Motility

, Volume 16, Issue 5, pp 491–498 | Cite as

Essential light chain of Drosophila nonmuscle myosin II

  • Kevin A. Edwards
  • Xiao-Jia Chang
  • Daniel P. Kiehart
Papers

Summary

We have cloned and sequenced a cDNA encoding the essential (alkaline) light chain of nonmuscle myosin from Drosophila melanogaster. The protein predicted from the cDNA matches partial amino acid sequence derived from essential light chain protein that copurifies with native nonmuscle myosin heavy chain. This completes the sequence of the three myosin subunits, two of which have been shown genetically to be required for morphogenesis and cytokinesis (the heavy chain encoded by zipper and the regulatory light chain encoded by spaghetti squash). The essential light chain protein is 147 amino acids in length and is 53% identical to human smooth muscle essential light chain. The sequence is consistent with the presence of four helix-loop-helix domains seen in crystallographic structures of the striated muscle myosin light chains and their close relative, calmodulin. We identified the most conserved residues among essential light chain sequences from multiple phyla and present their locations on the crystallographic structure of striated muscle essential light chain. This highlights several conserved contacts among the myosin subunits that may be important for the structure and regulation of the myosin motor. The gene encoding Drosophila nonmuscle essential light chain (Mlc-c) localizes to cytological position 5A6 and we discuss prospects for genetic analysis in this region.

Keywords

Striate Muscle Light Chain Heavy Chain Myosin Heavy Chain Myosin Light Chain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Kevin A. Edwards
    • 1
    • 2
  • Xiao-Jia Chang
    • 2
    • 3
  • Daniel P. Kiehart
    • 1
    • 2
  1. 1.Department of Cell BiologyDuke University Medical CenterDurhamUSA
  2. 2.Department of Cellular and Developmental Biology, Harvard Biological LaboratoriesHarvard UniversityCambridgeUSA
  3. 3.Genetics InstituteCambridgeUSA

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