Tissue reactions to lead samples in a late infection rat model

  • P. B. Van Wachem
  • M. J. A. Van Luyn
  • A. W. De Wit
  • D. Raatjes
  • M. L. P. M. Verhoeven
  • M. Hendriks
  • P. T. Cahalan


Tissue reactions to rat lead samples, modelling for clinically used leads, were investigated in a late infection model, in which injection of bacteria was performed after a 3-week encapsulation process. At the site of injection, detachment of the original fibrous capsule, wound fluid infiltration, fibrin formation and granulocyte and macrophage infiltrations, occurred. Spreading of infection did not occur via the generally assumed direct bacterial adhesion to materials, but through blood vessels at the outside of capsules and through wound fluid passage at the interface and in the lumen of the lead sample. At day 5, infection had spread all over, but, apart from two small abscesses, seemed to be suppressed at day 10. However, probably due to luminal bacterial growth, at weeks 3 and 6 the reaction intensified showing larger abscesses with accumulations of lymphocytes. The results of this study represent a good basis for further studies aimed at developing infection-resistent lead material. Research efforts are first directed on modification of material surfaces to provide controlled release of antimicrobial agents.


Luminal Encapsulation Late Infection Good Basis Tissue Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    J. BLACK, “Biological performance of materials: Fundamentals of biocompatibility”, 2nd Edn (Marcel Dekker, New York, 1992).Google Scholar
  2. 2.
    J. DANKERT, A. H. HOGT and J. FEIJEN, CRC Crit. Rev. Biocompat. 2 (1986) 219.Google Scholar
  3. 3.
    S. H. DOUGHERTY and R. L. SIMMONS. Curr. Probl. Surg. 19 (1982) 217.Google Scholar
  4. 4.
    F. J. SCHOEN, American Society for Artificial Organs, J. 33 (1987) 8.Google Scholar
  5. 5.
    A. F.VON RECUM and E. BARTH. J. Invest. Surg. 2 (1989) 351.Google Scholar
  6. 6.
    P. B.VAN WACHEM, M. J. A.VAN LUYN, E. H. BLAAUW, D. RAATJES, P. T. CAHALAN and M. HENDRIKS. J. Mater. Sci. Mater. Med. 5 (1994) 628.Google Scholar
  7. 7.
    A. E. KHOURY, K. LAM, B. ELLIS and J. W. COSTERTON. American Society for Artificial Organs, J 38 (1991) M174.Google Scholar
  8. 8.
    G. D. CHRISTENSEN, L. M. BADDOUR and W. A. SIMPSON. Zbl. Bakt. Suppl. 16 (1987) 103.Google Scholar
  9. 9.
    J. W. COSTERTON, Rev. Infect. Dis. 6 (suppl. 3) (1984) S608.Google Scholar
  10. 10.
    W. P. REED and R. C. WILLIAMS. J. Chron. Dis. 31 (1978) 67.Google Scholar
  11. 11.
    K. MERRITT, J. W. SHAFER and S. A. BROWN. J. Biomed. Mater. Res. 13 (1979) 101.Google Scholar
  12. 12.
    J. M. ANDERSON. Cardiovasc. Pathol. 2 (1993) 335.Google Scholar
  13. 13.
    F. A. WALDVOGEL. in “Principles and practice of infectious diseases”, 3rd Edn (Churchill Livingston, New York, 1990) pp. 1489–1511.Google Scholar
  14. 14.
    A. K. ABBAS, A. H. LICHTMAN and J. S. POBER. “Cellular and molecular immunology” (W. B. Saunders, Philadelphia, PA, 1991).Google Scholar
  15. 15.
    J. BROSTOFF, G. K. SCADDING, D. K. MALE and I. M. ROITT. “Clinical immunology” (Gower Medical Publishing, London, 1991).Google Scholar
  16. 16.
    J. B.VAN DER MEER and M. C. J. M.DE JONG. Neth. J. Med. 40 (1992) 244.Google Scholar
  17. 17.
    B. JANSEN, J. SCHIERHOLZ, F. SCHUMACHER-PERDREAU, G. PETERS and G. PULVERER. Adv. Biomater. 9 (1990) 117.Google Scholar
  18. 18.
    G. COLOMB and A. SHPIGELMAN. J. Biomed. Mater. Res. 25 (1991) 937.Google Scholar
  19. 19.
    B. JANSEN, F. SCHUMACHER-PERDREAU, G. PETERS and G. PULVERER, J. Inv. Surg. 2 (1989) 361.Google Scholar
  20. 20.
    A. G. GRISTINA. Science 237 (1987) 1588.Google Scholar

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • P. B. Van Wachem
    • 1
  • M. J. A. Van Luyn
    • 1
  • A. W. De Wit
    • 1
  • D. Raatjes
    • 2
  • M. L. P. M. Verhoeven
    • 3
  • M. Hendriks
    • 3
  • P. T. Cahalan
    • 3
  1. 1.Laboratory for Cell Biology and Electron MicroscopyGroningen UniversityGroningenThe Netherlands
  2. 2.Dept. of Hospital EpidemiologyUniversity HospitalGroningenThe Netherlands
  3. 3.Medtronic Bakken Research Center BVMaastrichtThe Netherlands

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