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Clinical & Experimental Metastasis

, Volume 13, Issue 5, pp 396–404 | Cite as

Inhibition of cancer cell motility and invasion by interleukin-12

  • Stephen Hiscox
  • Maurice B. Hallett
  • Malcom C. A. Puntis
  • Wen G. Jiang
Article

Tumour cell motility and attachment are crucial requirements in the formation of metastatic lesions. These properties are affected by a number of cytokines including hepatocyte growth factor/scatter factor (HGF/SF) and several immunoregulatory proteins, including interleukin-12 (IL-12). Although IL-12 has been reported to exhibit potent anti-tumour effects in vivo, a direct effect of IL-12 on cancer cells has not been reported. We show here that IL-12 directly inhibited the attachment of the human colon cancer cell lines HRT18, HT29 and HT115 to Matrigel, HGF/SF-stimulated cell motility and HGF/SF-induced cell invasion through a reconstituted basement membrane. IL-12 did not affect the growth of these cell lines. Flow cytometry, Western analysis and immunohistochemistry revealed an up-regulation of E-cadherin cell-surface adhesion molecules. These direct effects of IL-12 on colon cancer cells suggest a potentially important role for IL-12 in metastasis.

Keywords

cell motility colon cancer interleukin-12 invasion metastasis 

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Copyright information

© Rapid Science Publishers 1995

Authors and Affiliations

  • Stephen Hiscox
    • 1
  • Maurice B. Hallett
    • 1
  • Malcom C. A. Puntis
    • 1
  • Wen G. Jiang
    • 1
  1. 1.Department of SurgeryUniversity of Wales College of MedicineCardiffUK

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