Letters in Peptide Science

, Volume 2, Issue 3–4, pp 259–260

Screening of a synthetic pentapeptide library composed of d-amino acids against fructose-1,6-biphosphate aldolase

  • Isabelle Samson
  • Jef Rozenski
  • Arthur van Aerschot
  • Bart Samyn
  • Jozel van Beeumen
  • Piet Herdewijn
Section II. Synthesis and Libraries

Summary

A synthetic peptide library, theoretically composed of 537 824 d-amino acid pentapeptides anchored on polystyrene beads, was prepared with each bead bearing a single pentapeptide sequence. This library was screened for interaction with fructose-1,6-biphosphate aldolase of T. brucei labelled with biotin. Affinity beads that bound the enzyme were selected with streptavidin-coated magnetic beads. A total of 19 beads were isolated and individually subjected to Edman microsequence analysis. The corresponding peptide sequences were synthesized and evaluated for enzyme activity inhibition.

Keywords

d-amino acids Library Aldolase Screening Magnetic beads 

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Copyright information

© ESCOM Science Publishers B.V 1995

Authors and Affiliations

  • Isabelle Samson
    • 1
  • Jef Rozenski
    • 1
  • Arthur van Aerschot
    • 1
  • Bart Samyn
    • 2
  • Jozel van Beeumen
    • 2
  • Piet Herdewijn
    • 1
  1. 1.Laboratory of Medicinal Chemistry (F.F.W.), Rega Institute for Medical ResearchKatholieke Universiteit LeuvenLeuvenBelgium
  2. 2.Department of Biochemistry, Physiology and MicrobiologyRijks Universiteit GentGentBelgium

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