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Photosynthesis Research

, Volume 10, Issue 3, pp 303–308 | Cite as

Energy-dependent quenching of dark-level chlorophyll fluorescence in intact leaves

  • W. Bilger
  • U. Schreiber
II. Excitation and Energy Migration; Regulation of Energy Transfer; State Transition; and Variable Chlorohyll a Fluorescence

Abstract

A new type of modulation fluorometer was used in the study of energy-dependent chlorophyll fluorescence quenching (qE) in intact leaves. Under conditions of strong energization of the thylakoid membrane (high light intensity, absence of CO2) not only variable fluorescence, FV, but also dark-level fluorescence, FO, was quenched, leading to definition of a quenching coefficient, qO. Information on qO was shown to be essential for correct determination of photochemical (qQ) and energy dependent quenching (qE) by the saturation pulse method. The relationship between qE and qO was analysed over a range of light intensities at steady state conditions. qE was found to consist of two components, the second of which is linearly correlated with qO. qO and the second component of qE are interpreted to reflect the state 1 — state 2 shift caused by LHC II phosphorylation.

Keywords

Chlorophyll Light Intensity State Condition Steady State Condition Chlorophyll Fluorescence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Martinus Nijhoff Publishers 1986

Authors and Affiliations

  • W. Bilger
    • 1
    • 2
  • U. Schreiber
    • 1
    • 2
  1. 1.Institut für BotanikWürzburgFRG
  2. 2.Pharmazeutische Biologie der Universität WürzburgWürzburgFRG

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