Cell Biology and Toxicology

, Volume 1, Issue 4, pp 285–295 | Cite as

Enzyme leakage due to change of membrane permeability of primary cultured rat hepatocytes treated with various hepatotoxins and its prevention by glycyrrhizin

  • Toshikazu Nakamura
  • Takeru Fujii
  • Akira Ichihara

Various hepatotoxins were added to the medium of primary cultures of adult rat hepatocytes and the release of the cytosolic enzymes lactic dehydrogenase, glutamic-oxaloacetic and glutamic-pyruvic aminotransferases were measured 24 h later. CCl4 at low concentrations caused dose-dependent release of soluble enzymes into medium without appreciable cytolysis of the hepatocytes. Mitochondrial enzymes were not released under these conditions. At 5 mM CCl4, both soluble and mitochondrial glutamic-oxaloacetic aminotransferase were found in the culture medium. Glycyrrhizin, a triterpenoid glycoside of licorice roots, prevented the enzyme release caused by CCl4.

Key words

enzyme leakage glycyrrhizin hepatotoxins primary cultured hepatocytes 



carbon tetrachloride


glutamic dehydrogenase


glutamic-oxaloacetic aminotransferase


glutamic-pyruvic aminotransferase


lactic dehydrogenase


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Copyright information

© Princeton Scientific Publishing Co., Inc 1985

Authors and Affiliations

  • Toshikazu Nakamura
    • 1
  • Takeru Fujii
    • 1
  • Akira Ichihara
    • 1
  1. 1.Institute for Enzyme Research, School of MedicineUniversity of TokushimaTokushimaJapan

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