Plant Cell, Tissue and Organ Culture

, Volume 23, Issue 1, pp 39–44 | Cite as

Pigment and isozyme variation in aspen shoots regenerated from callus culture

  • Eun-Woon Noh
  • Subhash C. Minocha
Article
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Abstract

Pigment as well as isozyme variations were observed among aspen (Populus tremuloides Michx.) plants regenerated from callus cultures. Out of more than 600 plantlets, two chimeric plants (one with green base and two albino shoots and the other with an albino shoot) were produced. Callus derived from albino shoots produced albino as well as chimeric plants when transferred to shoot inducing medium. Isozyme patterns of 119 plants were examined by starch gel electrophoresis. Thirty plants showed variation in shikimic dehydrogenase isozyme and 41 in isocitric dehydrogenase. Variation was also observed in malate dehydrogenase and phosphoglucose isomerase. No variation was seen in 6-phosphogluconate dehydrogenase. Pigment variation was not associated with any isozyme changes.

Key words

albino aspen chimera callus culture somaclonal variation 

Abbreviations

BA

6-benzyladenine

IBA

indole-3-butyric acid

GD

Gresshoff & Doy medium

WPM

woody plant medium

SKD

shikimic dehydrogenase

IDH

isocitric dehydrogenase

MDH

malate dehydrogenase

PGI

phosphoglucose isomerase

6-PGD

6-phosphogluconate dehydrogenase

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Eun-Woon Noh
    • 1
  • Subhash C. Minocha
    • 1
  1. 1.Department of Plant BiologyUniversity of New HampshireDurhamUSA

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