Biodiversity & Conservation

, Volume 4, Issue 1, pp 1–9 | Cite as

Isolation and properties of barophilic and barotolerant bacteria from deep-sea mud samples

  • Chiaki Kato
  • Takako Sato
  • Koki Horikoshi


Several barophilic and barotolerant bacteria were isolated from deep-sea mud samples of Suruga Bay (2485 m depth), the Ryukyu Trench (5110 m depth), and the Japan Trench (land-side 6356 m, and sea-side 6269 m depth, respectivelys. The barophilic bacteria, strains DB5501, DB6101, DB6705 and DB6906, were albe to grow better under high hydrostatic pressures than under atmospheric pressure (0.1 megapascals; MPa). The optimal growth pressures for the barophilic bacteria were approximately 50 MPa at 10°C. The barotolerant strains DSK1 and DSS12 were determined to be psychrophilic, and had optimal growth temperatures of 10°C and 8°C, respectively. The degree of barophily and barotolerance was shown to be very dependent on temperature. For example, at 4°C the barophilic strains were indistinguishable from barotolerant bacteria, whereas at 15°C the barotolerant strains behaved more like the barophilic strains. Based on sequence analysis of 16S ribosomal DNA, all of the strains included in this study belong to the gamma subgroup of the Proteobacteria. Phylogenetic relations between the isolated strains and the known gamma subgroup bacteria suggested that the isolated strains belong to a new sub-branch of this group.

Key words

barophilic bacteria barotolerant bacteria deep-sea high pressure bacterial phylogeny 


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Copyright information

© Chapman & Hall 1995

Authors and Affiliations

  • Chiaki Kato
    • 1
  • Takako Sato
    • 1
  • Koki Horikoshi
    • 1
  1. 1.The DEEP STAR GroupJapan Marine Science and Technology CenterYokosukaJapan

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