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Photosynthesis Research

, Volume 19, Issue 1–2, pp 153–184 | Cite as

Recent developments in chloroplast protein transport

  • Michael L. Mishkind
  • Scott E. Scioli
Protein Transport Minireview

Abstract

Most proteins located in chloroplasts are encoded by nuclear genes, synthesized in the cytoplasm, and transported into the organelle. The study of protein uptake by chloroplasts has greatly expanded over the past few years. The increased activity in this field is due, in part, to the application of recombinant DNA methodology to the analysis of protein translocation. Added interest has also been gained by the realization that the transport mechanisms that mediate protein uptake by chloroplasts, mitochondria and the endoplasmic reticulum display certain characteristics in common. These include amino terminal sequences that target proteins to particular organelles, a transport process that is mechanistically independent from the events of translation, and an ATP-requiring transport step that is thought to involve partial unfolding of the protein to be translocated. In this review we examine recent studies on the binding of precursors to the chloroplast surface, the energy-dependent uptake of proteins into the stroma, and the targeting of proteins to the thylakoid lumen. These aspects of protein transport into chloroplasts are discussed in the context of recent studies on protein uptake by mitochondria.

Key words

mitochondria organelle biogenesis transit peptide chloroplast protein transport 

Abbrevlations

CAT

chloramphenicol acetyl transferase

CCCP

carbonylcyanide m-chlorophenylhydrazone

DHFR

dihydrofolate reductase

EPSP

5-enol-pyruvylshikimate-3-phosphate

ER

endoplasmic reticulum

LHCP

light harvesting chlorophyll a/b apoprotein

NPT

neomycin phosphotransferase

oATP

adenosine-2′,3′-dialdehyde-5′-triphosphate

P-inorganic phosphate Rubisco

ribulose-1,5-bisphosphate carboxylase/oxygenase

SSU

small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase

SRP

signal recognition particle

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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Michael L. Mishkind
    • 1
  • Scott E. Scioli
    • 1
  1. 1.Department of Biochemistry and MicrobiologyCook College, Rutgers UniversityNew BrunswickUSA

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