Cell Biology and Toxicology

, Volume 3, Issue 2, pp 109–111 | Cite as

Unscheduled DNA synthesis: Workshop overview

  • Daniel A. Casciano

Key words

autoradiography carcinogens hepatocyte scintillation counting xenobiotics 



unscheduled DNA synthesis


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  1. ASCLTHAUS, F.R., LAWRENCE, S.D., SATTLER, G.L., LONGFELLOW, D.G. and PITOT, H.C. (1982). Chemical quantification of unscheduled DNA synthesis in cultured hepatocytes as an assay for the rapid screening of potential chemical carcinogens. Cancer Res. 42:3010–3015.Google Scholar
  2. BONNEY, R.J. (1974). Adult liver parenchymal cells in primary culture: Characteristics and cell recognition standards. In Vitro 10:130–142.Google Scholar
  3. CASCIANO, D.A., FARR, J.A., OLDHAM, J.W. and CAVE, M.D. (1978). 2-Acetylaminofluorene-induced unscheduled DNA synthesis in hepatocytes isolated from 3methylcholanthrene treated rats. Cancer Lett. 5:173–178.Google Scholar
  4. CASCIANO, D.A. and GAYLOR, D.W. (1983). Statistical criteria for evaluating chemicals as positive or negative in the hepatocyte/ DNA repair assay. Mutation Res. 122:81–86.Google Scholar
  5. HOWARD, P.C., CASCIANO, D.A., BELAND, F.A. and SHADDOCK, J.G., JR. (1981). The binding of N-hydroxy-2-acetylaminofluorene to DNA and repair of the adducts of primary rat hepatocyte cultures. Carcinogenesis 2:97–102.Google Scholar
  6. LAISHES, B.A. and WILLIAMS, G.M. (1967a). Conditions affecting primary cultures of functional adult rat hepatocytes, I. The effect of insulin. In Vitro 12:521–532.Google Scholar
  7. LAISHES, B.A. and WILLIAMS, G.M. (1976b). Conditions affecting primary cell cultures of functional adult rat hepatocytes, II. Dexamethasome enhanced longevity and maintenance of morphology. In Vitro 12:821–832.Google Scholar
  8. LABREQUE, D.R. and HOWARD, R.B. (1976). The preparation and characterization of intact isolated parenchymal cells from rat liver. In: Methods in Cell Biology, Vol. XIV (D.M. Prescott, ed.), pp. 327–340. Academic Press, New York.Google Scholar
  9. MCQUEEN, C.A. and WILLIAMS, G.M. (1981). Characterization of DNA repair elicited by carcinogens and drugs in the hepatocyte primary culture/ DNA repair test. J. Toxicol. Environ. Hlth. 8:463–477.Google Scholar
  10. MICHALOPOULOS, G., SATTLER, G.L., O'CONNOR, L. and PITOT, H.C. (1978). Unscheduled DNA synthesis induced by procarcinogens in suspensions and primary cultures of hepatocytes on collagen membranes. Cancer Res. 38:1966–1971.Google Scholar
  11. PROBST, G.S., MCMAHON, R.E., HILL, E.E., THOMPSON, C.Z., EPP, J.K. and NEAL, S. B. (1981). Chemically-induced unscheduled DNA synthesis in primary rat hepatocyte cultures: A comparison with bacterial mutagenicity using 218 compounds. Environ. Mutagen. 3:11–32.Google Scholar
  12. SEGLEN, P.O. (1975). Preparation of isolated rat liver cells. In: Methods in Cell Biology, Vol. XIII (D.M. Prescott, ed.), pp. 29–83. Academic Press, New York.Google Scholar
  13. WILLIAMS, G.M. (1976). Carcinogen-induced DNA repair in primary rat liver cell cultures: A possible screen for chemical carcinogens. Cancer Lett. 1:231–236.Google Scholar
  14. WILLIAMS, G.M. (1977). The detection of chemical carcinogens by unscheduled DNA synthesis in rat liver primary cell cultures. Cancer Res. 37:1845–1851.Google Scholar
  15. WILLIAMS, G.M., BERMUDEZ, E. and SCARAMUZZINO, D. (1977). Rat hepatocyte primary cultures, III. Improved dissociation and attachment techniques and the enhancement of survival by culture medium. In Vitro 13:809–817.Google Scholar

Copyright information

© Princeton Scientific Publishing Co., Inc 1987

Authors and Affiliations

  • Daniel A. Casciano
    • 1
  1. 1.Division of Genetic ToxicologyNational Center for Toxicological ResearchJefferson

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