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Biodegradation

, Volume 7, Issue 1, pp 83–90 | Cite as

Effects of electron acceptors, reducing agents, and toxic metabolites on anaerobic degradation of heterocyclic compounds

  • Dorthe Licht
  • Birgitte K. Ahring
  • Erik Arvin
Regular Paper

Abstract

Degradation of four heterocyclic compounds was examined under nitrate-reducing, sulphate-reducing and methanogenic conditions. Soil samples from a creosote-polluted site in Denmark were used as inoculum. Indole and quinoline were degraded under all redox conditions with the highest degradation rates obtained under sulphate-reducing conditions. Benzothiophene and benzofuran were not degraded during the observation period of 100 days under any of the redox conditions. Indole and quinoline degrading cultures could be repeatedly transferred under all redox conditions, except for degradation of quinoline under sulphate-reducing conditions which was inhibited by sulphide at concentrations above 0.8 mM. Degradation of quinoline under methanogenic conditions was also inhibited by 3.2 mM sulphide used as a reducing agent, but sulphide had no inhibitory effect on the degradation of indole in methanogenic and sulphate-reducing soil slurries.

Key words

Anaerobic degradability creosote denitrification heterocyclic compounds methanogenesis reducing agents soil slurries sulphate-reduction sulphide inhibition 

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References

  1. Bak, F & Widdel, F (1986) Anaerobic degradation of indolic compounds by sulphate-reducing enrichment cultures, and description of Desulfobacterium indolicum gen. nov., sp. nov. Arch. Microbiol. 146: 170–176Google Scholar
  2. Berry, DF, Madsen, EL & Bollag, J-M (1987) Conversion of indole to oxindole under methanogenic conditions. Appl. Environ. Microbiol. 53: 180–182Google Scholar
  3. Godsy EM, Goerlitz DF & Grbić-Galić D (1992) Methanogenic degradation of heterocyclic aromatic compounds by aquifer-derived microcosms. In: Bioremediation of hazardous wastes, by Biosystems technology development program: 89–92Google Scholar
  4. Godsy EM & Grbić-Galić D (1988) Biodegradation pathways for benzothiophene in methanogenic microcosms. U.S. Geological Survey. Toxic Substances Hydrology Program: Proc. Technical Mett. Phoenix, September 26–30, 1988 (pp 559–564)Google Scholar
  5. Gu, J-D & Berry, DF (1991) Degradation of substituted indoles by an indole-degrading methanogenic consortium. Appl. Environ. Microbiol. 57: 2622–2627Google Scholar
  6. Kim, HY, Kim, TS & Kim, BH (1990) Degradation of organic sulphur compounds and the reduction of dibenzothiophene to biphenyl and hydrogen sulphide. Biotechnol. Lett. 12: 761–764Google Scholar
  7. Kuhn, EP & Suflita, JM (1989) Microbial degradation of nitrogen, oxygen and sulphur heterocyclic compounds under anaerobic conditions: Studies with aquifer samples. Environ. Toxicol. and Chem. 8: 1149–1158Google Scholar
  8. Jain MK, Zeikus JG & Bhatnagar L (1991) Methanogens. In: Levett PN (Ed) Anaerobic Microbiology. The practical approach series (pp 223–246) Series editors: Rickwood, D. & Hames, B.D. IRL Press, Oxford University PressGoogle Scholar
  9. Liu, SM, Jones, WJ & Rogers, JE (1994) Influence of redox potential on the anaerobic biotransformation of nitrogen-heterocyclic compounds in anoxic freshwater sediments. Appl. Microbiol. Biotechnol. 41: 717–724Google Scholar
  10. Madsen, T & Aamand, J (1991) Effects of sulfuroxy anions on degradation of pentachlorophenol by a methanogenic enrichment culture. Appl. Environ. Microbiol. 57: 2453–2458Google Scholar
  11. Madsen, EL & Bollag, J-M (1989) Pathway of indole metabolism by a denitrifying microbial community. Arch Microbiol 151: 71–76Google Scholar
  12. Madsen, EL, Fransis, AJ & Bollag, J-M (1988) Environmental factors affecting indole metabolism under anaerobic conditions. Appl. Environ. Microbiol. 54: 74–78Google Scholar
  13. Maka A, McKinley VL & Conrad JR (1987) Degradation of benzothiophene and dibenzothiophene under anaerobic conditions by mixed cultures. 87th Annual Meeting of the American Society for Microbiology, Georgia, March 5, 1987Google Scholar
  14. Mueller, JG, Chapman, PJ & Pritchard, PH (1989) Creosote-contaminated sites. Environ. Sci. Technol. 23: 1197–1201Google Scholar
  15. Pereira, WE, Rostad, CE, Updegraff, DM & Bennett, JL (1987) Anaerobic microbial transformation of azaarenes in ground water at hazardous-waste sites. Chemical Quality of Water and the Hydrologic Cycle. In: Averett, RC & McKnight, DM (Eds) (pp 111–123) Lewis Publishers, Inc., Michigan, USAGoogle Scholar
  16. Pereira, WE, Rostad, CE, Leiker, TJ, Updegraff, DM & Bennett, JL (1988) Microbial hydroxylation of quinoline in contaminated groundwater: Evidence for incorporation of the oxygen atom of water. Appl. Environ. Microbiol. 54: 827–829Google Scholar
  17. Reis, MAM, Almeida, JS, Lemos, PC & Carrondo, MJT (1992) Effect of hydrogen sulphide on growth of sulphate reducing bacteria. Biotechnology and Bioengineering 40: 593–600Google Scholar
  18. Shranker, R & Bollag, J-M (1990) Transformation of indole by methanogenic and sulphate-reducing microorganisms isolated from digested sludge. Microb. Ecol. 20: 171–183Google Scholar
  19. Wang, Y-T, Suidan, MT & Pfeffer, JT (1984) Anaerobic degradation of indole to methane. Appl. Environ. Microbiol. 48: 1058–1060Google Scholar
  20. Zehnder, AJB & Wuhrmann, K (1976) Titanium(III) citrate as a nontoxic oxidation-reduction buffering system for the culture of obligate anaerobes. Science 194: 1165–1166Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Dorthe Licht
    • 1
  • Birgitte K. Ahring
    • 1
  • Erik Arvin
    • 1
  1. 1.Institute of Environmental Science and Engineering, Groundwater Research CentreTechnical University of DenmarkLyngbyDenmark

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