Violaxanthin de-epoxidase in etiolated leaves
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In etiolated leaves the occurrence of the enzymatic violaxanthin de-epoxidation to zeaxanthin is shown. The carotenoid transformation is provoked by the infiltration of whole leaves with ascorbate at pH 5 and is susceptible to DTT. Identification of the de-epoxidase activity is achieved by in vivo spectroscopy and pigment analysis (TLC).
Key wordsCarotenoid transformation chloroplast development etiolated leaf in vivo spectroscopy xanthophyll cycle
N-2-hydroxyethylpiperazine-N′-2 ethanesulfonic acid
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