Photosynthesis Research

, Volume 15, Issue 1, pp 67–73

Violaxanthin de-epoxidase in etiolated leaves

  • Erhard Pfündel
  • Reto J. Strasser
Regular Paper

Abstract

In etiolated leaves the occurrence of the enzymatic violaxanthin de-epoxidation to zeaxanthin is shown. The carotenoid transformation is provoked by the infiltration of whole leaves with ascorbate at pH 5 and is susceptible to DTT. Identification of the de-epoxidase activity is achieved by in vivo spectroscopy and pigment analysis (TLC).

Key words

Carotenoid transformation chloroplast development etiolated leaf in vivo spectroscopy xanthophyll cycle 

Abbreviations

DTT

Dithiothreitol

Hepes

N-2-hydroxyethylpiperazine-N′-2 ethanesulfonic acid

TLC

Thin-layer Chromatography

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Copyright information

© Martinus Nijhoff Publishers 1988

Authors and Affiliations

  • Erhard Pfündel
    • 1
  • Reto J. Strasser
  1. 1.Institute of Biology, Department of BioenergeticsUniversity of StuttgartStuttgart 60Federal Republic of Germany

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