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Photosynthesis Research

, Volume 28, Issue 3, pp 149–153 | Cite as

Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach

  • Peter J. van Leeuwen
  • Maaike C. Nieveen
  • Erik Jan van de Meent
  • Jan P. Dekker
  • Hans J. van Gorkom
Rapid Communication

Abstract

Pure and active oxygen-evolving PS II core particles containing 35 Chl per reaction center were isolated with 75% yield from spinach PS II membrane fragments by incubation with n-dodecyl-β-D-maltoside and a rapid one step anion-exchange separation. By Triton X-100 treatment on the column these particles could be converted with 55% yield to pure and active PS II reaction center particles, which contained 6 Chl per reaction center.

Keywords

photosystem II core reaction center isolation 

Abbreviations

Bis-Tris

bis[2-hydroxyethyl]imino-tris[hydroxymethyl]methane

Chl

chlorophyll

CP29

Chl a/b protein of 29 kDa

Cyt b559

cytochrome b559

DCBQ

2,5-dichloro-p-benzo-quinone

LHC II

light-harvesting complex II, predominant Chl a/b protein

MES

2-[N-Morpholino]ethanesulfonic acid

Pheo

pheophytin

PS H

photosystem II

QA

bound plastoquinone, serving as the secondary electron acceptor in PS II (after Pheo)

SDS

sodiumdodecylsulfate

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Peter J. van Leeuwen
    • 1
  • Maaike C. Nieveen
    • 1
  • Erik Jan van de Meent
    • 1
  • Jan P. Dekker
    • 2
  • Hans J. van Gorkom
    • 1
  1. 1.Department of BiophysicsHuygens laboratory of the State UniversityLeiden
  2. 2.Department of Physics and AstronomyFree UniversityAmsterdamthe Netherlands

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