Photosynthesis Research

, Volume 28, Issue 3, pp 149–153 | Cite as

Rapid and simple isolation of pure photosystem II core and reaction center particles from spinach

  • Peter J. van Leeuwen
  • Maaike C. Nieveen
  • Erik Jan van de Meent
  • Jan P. Dekker
  • Hans J. van Gorkom
Rapid Communication


Pure and active oxygen-evolving PS II core particles containing 35 Chl per reaction center were isolated with 75% yield from spinach PS II membrane fragments by incubation with n-dodecyl-β-D-maltoside and a rapid one step anion-exchange separation. By Triton X-100 treatment on the column these particles could be converted with 55% yield to pure and active PS II reaction center particles, which contained 6 Chl per reaction center.


photosystem II core reaction center isolation 







Chl a/b protein of 29 kDa

Cyt b559

cytochrome b559




light-harvesting complex II, predominant Chl a/b protein


2-[N-Morpholino]ethanesulfonic acid




photosystem II


bound plastoquinone, serving as the secondary electron acceptor in PS II (after Pheo)




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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Peter J. van Leeuwen
    • 1
  • Maaike C. Nieveen
    • 1
  • Erik Jan van de Meent
    • 1
  • Jan P. Dekker
    • 2
  • Hans J. van Gorkom
    • 1
  1. 1.Department of BiophysicsHuygens laboratory of the State UniversityLeiden
  2. 2.Department of Physics and AstronomyFree UniversityAmsterdamthe Netherlands

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