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Plant Cell, Tissue and Organ Culture

, Volume 41, Issue 2, pp 193–196 | Cite as

Preliminary studies of micropropagation of Hopea odorata, a dipterocarp tree

  • E. S. Scott
  • A. N. Rao
  • C. S. Loh
Research Note

Abstract

Plantlet development from in vitro cultures of Hopea odorato Roxb. is described. Embryos excised from seeds and cultured on Gamborg's B5 or modified Murashige and Skoog (MS) medium with benzyladenine (BA, 2.2–22.2 μM) produced axillary shoots at cotyledonary and/or stem nodes. Shoot production was greatest in germinated embryos on modified MS medium with 8.9 μM BA. Excised axillary shoots formed few buds when cultured on medium with BA and limited root development occurred on Woody Plant Medium with naphthaleneacetic acid. Nodal explants from aseptically grown plantlets sprouted axillary shoots in modified MS medium with BA.

Key words

Dipterocarpaceae tissue culture 

Abbreviations

BA

6-benzyladenine

2,4-d

2,4-dichlorophenoxyacetic acid

GA3

gibberellic acid

IBA

indolebutyric acid

NAA

naphthaleneacetic acid

PVPP

polyvinylpolypyrrolidone

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • E. S. Scott
    • 1
  • A. N. Rao
    • 1
  • C. S. Loh
    • 1
  1. 1.Department of BotanyNational University of SingaporeSingapore

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