Experimental & Applied Acarology

, Volume 18, Issue 1, pp 51–59 | Cite as

A shared epitope found in the major paralysis inducing tick species of Africa

  • J. C. Crause
  • S. van Wyngaardt
  • R. Gothe
  • A. W. H. Neitz


Cross-reactivity between all the paralysis inducing tick species of veterinary relevance in Africa was demonstrated, by using a monoclonal antibody directed against the paralysis inducing toxin of Rhipicephalus evertsi evertsi. Western blot results, together with amino acid composition studies indicated that this monoclonal antibody recognizes protein bands of similar molecular mass and amino acid composition in R. evertsi evertsi and Ixodes rubicundus. This suggests that the Karoo paralysis toxin of I. rubicundus is possibly also a trimer with a high degree of homology to the spring lamb paralysis toxin of R. evertsi evertsi. The conclusive identity of these protein bands of 1. rubicundus could not be shown. Bio-assay studies performed on 1-day-old chickens suggested that the anti-spring lamb paralysis toxin monoclonal antibody also recognizes the paralysis toxin present in Argas (Persicargas) walkerae, by rendering some degree of protection against the effect of this toxin.


Western Blot Monoclonal Antibody Molecular Mass Acid Composition Protein Band 
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Copyright information

© Science and Technology Letters 1993

Authors and Affiliations

  • J. C. Crause
    • 1
  • S. van Wyngaardt
    • 1
  • R. Gothe
    • 2
  • A. W. H. Neitz
    • 1
  1. 1.Department of BiochemistryUniversity of PretoriaPretoriaRepublic of South Africa
  2. 2.Institute for Comparative Tropical Medicine and ParasitologyUniversity of MunichMunich 40Germany

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