Plant Molecular Biology

, Volume 30, Issue 3, pp 679–684

Requirement of a CCGAC cis-acting element for cold induction of the BN115 gene from winter Brassica napus

  • Chao Jiang
  • Betty Iu
  • Jas Singh
Short Communication

DOI: 10.1007/BF00049344

Cite this article as:
Jiang, C., Iu, B. & Singh, J. Plant Mol Biol (1996) 30: 679. doi:10.1007/BF00049344

Abstract

Mutation of the core pentamer, CCGAC, of two putative low temperature responsive elements (LTREs) in the 5′-proximal region of the winter Brassica napus cold-induced gene BN115 was carried out. Analyses of transient expression of the resultant mutated BN115 promoter-GUS fusions revealed the loss of low-temperature regulation by the promoter. This indicates that the CCGAC sequence is critical to the low-temperature response in the BN115 gene. In contrast, mutation of two G-boxes, CACGTG, staggered between the LTREs in the same region of the promoter did not alter cold-inducible gene expression. Replacement of a possible enhancer region of the BN115 promoter with the enhancer from the CaMV 35S promoter resulted in a several-fold increase in low temperature-induced GUS activity.

Key words

Brassica napus cis-acting element cold-inducible gene enhancer G-box LTRE 

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Chao Jiang
    • 1
  • Betty Iu
    • 1
  • Jas Singh
    • 1
  1. 1.Central Experimental FarmPlant Research Centre, Agriculture CanadaOttawaCanada

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