Advertisement

Plant Molecular Biology

, Volume 30, Issue 3, pp 647–653 | Cite as

Molecular cloning of a cDNA encoding diacylglycerol kinase (DGK) in Arabidopsis thaliana

  • Takeshi Katagiri
  • Tsuyoshi Mizoguchi
  • Kazuo Shinozaki
Short Communication

Abstract

Diacylglycerol kinase (DGK) synthesizes phosphatidic acid from diacylglycerol, an activator of protein kinase C (PKC), to resynthesize phosphatidylinositols. The structure of DGK has not been characterized in plants. We report the cloning of a cDNA, cATDGK1, encoding DGK from Arabidopsis thaliana. The cATDGK1 cDNA contains an open reading frame of 2184 bp, and encodes a putative protein of 728 amino acids with a predicted molecular mass of 79.4 kDa. The deduced ATDGK1 amino acid sequence exhibits significant similarity to that of rat, pig, and Drosophila DGKs. The ATDGK1 mRNA was detected in roots, shoots, and leaves. Southern blot analysis suggests that the ATDGK1 gene is a single-copy gene. The existence of DGK as well as phospholipase C suggests the existence of PKC in plants.

Key words

Arabidopsis thaliana diacylglycerol kinase EF-hand cysteine-rich zinc finger PI turnover Ca2+-binding proteins signal transduction pathways plants 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Alexandre J, Lassalles JP, Kado RT: Opening of Ca2+ channels in isolated red beet root vacuole membrane by inositol 1,4,5-triphosphate. Nature 343: 567–570 (1990).Google Scholar
  2. 2.
    Babu YS, Sack JS, Greenhough TJ, Bugg CE, Means AR, Cook WJ: Three-dimensional structure of calmodulin. Nature 315: 37–40 (1985).Google Scholar
  3. 3.
    Burns DJ, Bell RM: Protein kinase C contains two phorbol ester binding domains. J Biol Chem 266: 18330–18398 (1991).Google Scholar
  4. 4.
    Drøbak BK, Ferguson IB: Release of Ca2+ from plant hypocotyl microsomes by inositol-1,4,5-triphosphate. Biochem Biophys Res Commun 130: 1241–1246 (1985).Google Scholar
  5. 5.
    Ettlinger C, Lehle L: Auxin induces rapid changes in phosphatidylinositol metabolites. Nature 331: 176–178 (1988).Google Scholar
  6. 6.
    Goto K, Funayama M, Kondo H: Cloning and expression of a cytoskeleton-associated diacylglycerol kinase that is dominantly expressed in cerebellum. Proc Natl Acad Sci USA 91: 13042–13046 (1994).Google Scholar
  7. 7.
    Harper JF, Binder BM, Sussman MR: Calcium and lipid regulation of an Arabidopsis protein kinase expressed in Escherichia coli. Biochemistry 32: 3282–3290 (1993).Google Scholar
  8. 8.
    Hirayama T, Ohto C, Mizoguchi T, Shinozaki K: A gene encoding phosphatidylinositol-specific phospholipase C in induced by dehydration and salt stress in Arabidopsis thaliana. Proc Natil Acad Sci USA 92: 3903–3907 (1995).Google Scholar
  9. 9.
    Kamada Y, Muto S: Ca2+ regulation of phosphatidylinositol turnover in the plasma membrane of tobacco suspension culture cells. Biochim Biophys Acta 1093: 72–79 (1991).Google Scholar
  10. 10.
    Kanoh H, Yamada K, Sakane F: Diacylglycerol kinase: a key modulator of signal transduction? Trends Biochem Sci 15: 47–50 (1990).Google Scholar
  11. 11.
    Knight MR, Campbell AK, Smith SM, Trewavas AJ: Transgenic plant aequorin reports the effects of touch and cold-shock and elicitors on cytoplasmic calcium. Nature 352: 524–526 (1991).Google Scholar
  12. 12.
    Knight MR, Smith SM, Trewavas AJ: Wind-induced plant motion immediately increases cytosolic calcium. Proc Natl Acad Sci USA 89: 4967–4971 (1992).Google Scholar
  13. 13.
    Masai I, Okazaki A, Hosoya T, Hotta Y: Drosophila retinal degeneration A gene encodes an eye-specific diacylglycerol kinase with cysteine-rich zinc-finger motifs and ankyrin repeats. Proc Natl Acad Sci USA 90: 11157–11161 (1993).Google Scholar
  14. 14.
    Mizoguchi T, Yamaguchi-Shinozaki K, Hayashida N, Kamada H, Shinozaki K: Cloning and Characterization of two cDNAs encoding casein kinase II catalytic subunits in Arabidopsis thaliana. Plant Mol Biol 21: 279–289 (1993).Google Scholar
  15. 15.
    Moolenaar WH, Kruijer W, Tilly BC, Verlaan I, Bierman AJ, de Laat SW: Growth factor-like action of phosphatidic acid. Nature 323: 171–173 (1986).Google Scholar
  16. 16.
    Murayama T, Ui M: Phosphatidic acid may stimulate membrane receptors mediating adenylate cyclase inhibition and phospholipid breakdown in 3T3 fibroburasts. J Biol Chem 262: 5522–5529 (1987).Google Scholar
  17. 17.
    Ono Y, Fujii T, Igarashi K, Kikkawa U, Ogita K, Nishizuka Y: Nucleotide sequences of cDNAs for alpha and gamma subspecies of rat brain protein kinase C. Nucl Acids Res 16: 5199–5200 (1988).Google Scholar
  18. 18.
    Osada S, Mizuno K, Saido TC, Akita Y, Suzuki K, Kuroki T, Ohno S: A phorbol ester receptor/protein kinase, nPKCη, a new member of the protein kinase C family predominantly expressed in lung and skin. J Biol Chem 265: 22434–22440 (1990).Google Scholar
  19. 19.
    Perera IY, Zielinski RE: Structure and expression of the Arabidopsis CaM-3 calmodulin gene. Plant Mol Biol 19: 649–664 (1992).Google Scholar
  20. 20.
    Ranjeva R, Carrasco A, Boudet AM: Inositol triphosphate stimulates the release of calcium from intact vacuoles isolated from Acer cells. FEBS Lett 230: 137–141 (1988).Google Scholar
  21. 21.
    Reddy ASN, Poovaiah BW: Inositol 1,4,5-triphosphate induced calsium release from corn coleoptile microsomes. J Biochem 101: 569–573 (1987).Google Scholar
  22. 22.
    Sakane F, Imai S, Yamada K, Kanoh H: The regulatory role of EF-hand motifs of pig 80k diacylglycerol kinese as assessed using truncation and deletion mutants. Biochem Biophys Res Commun 181: 1015–1021 (1991).Google Scholar
  23. 23.
    Sakane F, Yamada K, Imai S, Kanoh H: Porcine 80-kDa diacylglycerol kinase is a calcium-binding and calcium/phospholipid-dependent enzyme and undergoes calcium-dependent translocation. J Biol Chem 266: 7096–7100 (1991).Google Scholar
  24. 24.
    Sakane F, Yamada K, Kanoh H, Yokoyama C, Tanabe T: Porcine diacylglycerol kinase sequence has zinc finger and E-F hand motifs. Nature 344: 345–348 (1990).Google Scholar
  25. 25.
    Sambrook J, Fritsch E, Maniatis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1989).Google Scholar
  26. 26.
    Schaap D, van der Wal J, van Blitterswijk WJ, van der Bend RL, Ploegh HL: Diacylglycerol kinase is phosphorylated in vivo upon stimulation of the epidermal growth factor receptor and serine/threonine kinases, including protein kinase Cɛ. Biochem J 289: 875–881 (1993).Google Scholar
  27. 27.
    Schumaker KS, Sze H: Inositol 1,4,5-triphosphate release Ca2+ from vacuolar membrane vesicles of oat roots. J Biol Chem 262: 3944–3946 (1987).Google Scholar
  28. 28.
    Smith DB, Johnson KS: Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67: 31–40 (1988).Google Scholar
  29. 29.
    Tufty RM, Kretsinger RH: Troponin and parvalbumin calcium binding regions predicted in myosin light chain and T4 lysozyme. Science 187: 167–169 (1975).Google Scholar
  30. 30.
    Urao T, Katagiri T, Mizoguchi T, Yamaguchi-Shinozaki K, Hayashida N, Shinozaki K: An Arabidopsis thaliana cDNA encoding Ca2+-dependent protein kinase. Plant Physiol 105: 1461–1462 (1994).Google Scholar
  31. 31.
    Urao T, Katagiri T, Mizoguchi T, Yamaguchi-Shinozaki K, Hayashida N, Shinozaki K: Two genes that encode Ca2+-dependent protein kinases are induced by drought and high-salt stresses in Arabidopsis thaliana. Mol Gen Genet 244: 331–340 (1994).Google Scholar
  32. 32.
    Wissing J, Heim S, Wagner KG: Diacylglycerol kinase from suspension cultured plant cells: purification and properties. Plant Physiol 90: 1546–1551 (1989).Google Scholar
  33. 33.
    Yamamoto YT, Conkling MA, Sussex IM, Irish VF: An Arabidopsis cDNA related to animal phoshoinositide-specific phospholipase C genes. Plant Physiol 107: 1029–1030 (1995).Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Takeshi Katagiri
    • 1
    • 2
  • Tsuyoshi Mizoguchi
    • 1
  • Kazuo Shinozaki
    • 1
    • 2
  1. 1.Laboratory of Plant Molecular Biology, Institute of Physical and Chemical Research (RIKEN)Tsukuba Life Science CenterTsukuba, IbarakiJapan
  2. 2.Institute of Biological SciencesUniversity of TsukubaTsukuba, IbarakiJapan

Personalised recommendations