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Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm

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Abstract

Shoot tips and leafy bud fragments removed from offshoots of adult date palms (Phoenix dactylifera L.) were cultured on a nutrient medium containing the Murashige and Skoog inorganic salts, 453 μM 2,4-dichlorophenoxyacetic acid, 14.8 μM N6-(2-isopentenyl)adenine and 3 g l-1 activated charcoal to develop nodular callus after 8 months of culture. Callus was cultured in agar-solidified and stationary or shaken liquid media containing half-strength MS inorganic salts, 3 g l-1 activated charcoal and different sucrose concentrations to study the influence of these factors on somatic embryogenesis. The best conditions for embryo development were culturing in liquid medium shaken at 100 rpm for a period of 2 weeks without sucrose, followed by culture on 3% sucrose.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

2iP:

N6-(2-isopentenyl) adenine

MS:

Murashige & Skoog (1962)

rpm:

revolutions per minute

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Authors and Affiliations

  1. Departamento de Cultivo de Tejidos, Instituto Valenciano de Investigaciones Agrarias, Apartado Oficial, 46113 Moncada, Valencia, Spain

    J. Veramendi & L. Navarro

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  1. J. Veramendi
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  2. L. Navarro
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Veramendi, J., Navarro, L. Influence of physical conditions of nutrient medium and sucrose on somatic embryogenesis of date palm. Plant Cell Tiss Organ Cult 45, 159–164 (1996). https://doi.org/10.1007/BF00048760

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  • DOI: https://doi.org/10.1007/BF00048760

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