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Plant Cell, Tissue and Organ Culture

, Volume 36, Issue 1, pp 141–143 | Cite as

Callus induction and shoot regeneration in Sempervivum tectorum

  • Éva Dobos
  • Béla Dános
  • Ábel László-Bencsik
Research Note

Abstract

Leaf and shoot explants of Sempervivum tectorum L., taken from 14- and 30-day-old plants germinated in vitro, have been studied by using Murashige-Skoog and White basal media with cytokinins (benzyladenine, kinetin) and auxins (indoleacetic acid, naphthaleneacetic acid, indolebutyric acid) in various concentrations. Explants taken from 14-day-old plants died but 30-day-old leaves and shoots produced yellow and soft, as well as green and hard calluses on Murashige-Skoog medium with 4.4–8.8 μM benzyladenine and 0.57 μM indoleacetic acid. Shoot organogenesis was induced from green, hard callus in a medium with 2.2 μM benzyladenine plus either 1.1 μM indoleacetic acid or 2.5 μM indolebutyric acid. Whole plants were grown on Murashige-Skoog medium without plant growth regulators. On the other hand, White medium was not suitable for raising Sempervivum tectorum in vitro.

Key words

medicinal plants organogenesis tissue culture 

Abbreviations

BA

benzyladenine

IAA

indoleacetic acid

IBA

indolebutyric acid

MS

Murashige-Skoog

NAA

napthaleneacetic acid

W

White

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Éva Dobos
    • 1
  • Béla Dános
    • 1
  • Ábel László-Bencsik
    • 1
  1. 1.Research Institute for Medicinal PlantsBudakalászHungary

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