Callus induction and shoot regeneration in Sempervivum tectorum
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Leaf and shoot explants of Sempervivum tectorum L., taken from 14- and 30-day-old plants germinated in vitro, have been studied by using Murashige-Skoog and White basal media with cytokinins (benzyladenine, kinetin) and auxins (indoleacetic acid, naphthaleneacetic acid, indolebutyric acid) in various concentrations. Explants taken from 14-day-old plants died but 30-day-old leaves and shoots produced yellow and soft, as well as green and hard calluses on Murashige-Skoog medium with 4.4–8.8 μM benzyladenine and 0.57 μM indoleacetic acid. Shoot organogenesis was induced from green, hard callus in a medium with 2.2 μM benzyladenine plus either 1.1 μM indoleacetic acid or 2.5 μM indolebutyric acid. Whole plants were grown on Murashige-Skoog medium without plant growth regulators. On the other hand, White medium was not suitable for raising Sempervivum tectorum in vitro.
Key wordsmedicinal plants organogenesis tissue culture
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