Plant Cell, Tissue and Organ Culture

, Volume 36, Issue 1, pp 141–143 | Cite as

Callus induction and shoot regeneration in Sempervivum tectorum

  • Éva Dobos
  • Béla Dános
  • Ábel László-Bencsik
Research Note


Leaf and shoot explants of Sempervivum tectorum L., taken from 14- and 30-day-old plants germinated in vitro, have been studied by using Murashige-Skoog and White basal media with cytokinins (benzyladenine, kinetin) and auxins (indoleacetic acid, naphthaleneacetic acid, indolebutyric acid) in various concentrations. Explants taken from 14-day-old plants died but 30-day-old leaves and shoots produced yellow and soft, as well as green and hard calluses on Murashige-Skoog medium with 4.4–8.8 μM benzyladenine and 0.57 μM indoleacetic acid. Shoot organogenesis was induced from green, hard callus in a medium with 2.2 μM benzyladenine plus either 1.1 μM indoleacetic acid or 2.5 μM indolebutyric acid. Whole plants were grown on Murashige-Skoog medium without plant growth regulators. On the other hand, White medium was not suitable for raising Sempervivum tectorum in vitro.

Key words

medicinal plants organogenesis tissue culture 





indoleacetic acid


indolebutyric acid




napthaleneacetic acid




Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brandao J & Salema R (1977) Callus and plantlets development from cultured leaf explants of Sedum telephium L. Z. Pflanzenphysiol. 85: 1–8Google Scholar
  2. Dudits D (1982) Callus cultures. In: Dudits D (Ed) Cell Fusion and Hybrid Plants (pp 21–24). Academic Press, BudapestGoogle Scholar
  3. Evans DA, Sharp WR & Flick CE (1981) Growth and behavior of cell cultures. In: Thorpe TA (Ed) Plant Tissue Culture: Methods and Application in Agriculture (pp 45–49). Academic Press, New YorkGoogle Scholar
  4. Kolb W & Schwarz T (1984) Properties and costs of substrates for extensive covering of flat roofs with vegetation. Gartenamt 33:2 83–90Google Scholar
  5. Kukhta EP, Aleksandrova IV & Paukov VN (1988) Polysac-charides from plant tissue cultures I. Properties and partial structure. Khim Prir Soedin Tashk 3: 323–346Google Scholar
  6. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497Google Scholar
  7. Müssel H & Kiermeier P (1983) Experience with xeromorphic plants for extensive green cover. Gartenamt 32: 6 376–381Google Scholar
  8. Thomas DA & André M (1987) Oxygen and carbon dioxide exchanges in Crassulacean acid metabolism plants: I. Effect of water stress on hourly and daily patterns. Plant Physiol. Biochem. 25:2 85–93Google Scholar
  9. Uhring J (1983) In vitro propagation of Sedum and Myrtus cultivars. HortScience 18: 616Google Scholar
  10. White PR (1943) Nutrient deficiency studies and an improved inorganic nutrient for cultivation of excised tomato roots. Growth 7: 53–65Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Éva Dobos
    • 1
  • Béla Dános
    • 1
  • Ábel László-Bencsik
    • 1
  1. 1.Research Institute for Medicinal PlantsBudakalászHungary

Personalised recommendations