Plant Cell, Tissue and Organ Culture

, Volume 36, Issue 1, pp 45–51 | Cite as

A preliminary evaluation of the use of microbial culture filtrates for the control of contaminants in plant tissue culture systems

  • S. Hussain
  • S. D. Lane
  • D. N. Price
Article

Abstract

The culture extracts of 21 micro-organisms were screened in vitro for anti-microbial activity against a range of test contaminants isolated from plant tissue cultures. Extracts produced by Trichoderma viride Pers. (strain A) and Bacillus subtilis Ehrenberg (strain 2) had the highest antifungal activity and extracts from Pseudomonas fluorescens Migula (strain X) had the highest antibacterial activity. When extracts of these organisms were applied to Nicotiana tabacum L. cultures, they reduced the growth of accidental contaminants. In the case of B. subtilis and P. fluorescens strains, equivalent extracts did not reduce fresh weight yields of uncontaminated N. tabacum cultures over four growth passages.

Key words

callus contamination control 

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References

  1. Baker CJ, Stavely JR & Morton M (1985) Biocontrol of bean rust by Bacillus subtilis under field conditions. Plant Dis. 69: 770–772Google Scholar
  2. Baker KF & Cook RJ (1974) Biological Control of Plant Pathogens. Freeman Press, San FranciscoGoogle Scholar
  3. Barath Z, Betina V & Nemec P (1964) Preparation of crude antibiotic concentrates. J. Antibiotic. 17: 144Google Scholar
  4. Barnett HL (1964) Mycoparasitism. Mycologia 56: 1–19Google Scholar
  5. Bauer AW, Kirby WMM, Sherris JC & Truck M (1966) Antibiotic susceptibility testing by a standardised single disc method. Amer. J. Clin. Pathol. 45: 493–496Google Scholar
  6. Becker JO & Hedges RW (1986) The production of antifungal compounds by phytosphere bacteria. Meded. Fac. Landbouwwet Rijksuniv. Gent. 50 (3b): 1135–1140Google Scholar
  7. Campbell R (1989) Biological Control of Microbial Plant Pathogens (pp 144–148). Cambridge University PressGoogle Scholar
  8. Chet I & Baker R (1981) Isolation and biocontrol potential of Trichoderma hamatum from soil naturally suppressive to Rhizoctonia solani. Phytopathology 17: 994–998Google Scholar
  9. Constantine DR (1986) Micropropagation in the commercial environment. In: Withers LA & Alderson PG (Eds) Plant Tissue Culture and its Agricultural Applications (pp 175–186). Butterworth, LondonGoogle Scholar
  10. Denis C & Webster J (1971) Antagonistic properties of species groups of Trichoderma. I. Production of non-volatile antibiotics. Trans. Brit. Mycol. Soc. 57: 25–39Google Scholar
  11. Dodds JH & Roberts LW (1981) Some inhibitory effectors on gentamicin on plant tissue cultures. In Vitro. 17: 467–470Google Scholar
  12. Falkiner FR (1990) The criteria for choosing an antibiotic for control of bacteria in plant tissue culture. Intl. Assoc. Plant Tiss. Cult. Newsl. 60: 13–23Google Scholar
  13. Herman EB (1987) New approaches to control of plant tissue culture contamination. Agricell Rep. 9: 33Google Scholar
  14. Kope HH & Fortin JA (1989) Antimicrobial compounds in the cell-free culture media of ectomycorrhizal fungi. New Phytol. 113: 57–63Google Scholar
  15. Leifert C, Waites WM & Nicholas JR (1989) Bacterial contaminants of micropropagated plant cultures. J. Applied Bacteriol. 67: 353–361Google Scholar
  16. Morris RAC & Lane SD (1990) Further observations on the interaction between Trichoderma viride and three Botrytis species. Mycologist 4: 195–197Google Scholar
  17. Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497Google Scholar
  18. Pollack K, Barfield DG & Shields R (1983) The toxicity of antibiotics to plant cell cultures. Plant Cell Rep. 2: 36–39Google Scholar
  19. Roulston S & Lane SD (1988) Observations on the interactions between Trichoderma viride and three Botrytis species. Mycologist 2: 176–177Google Scholar
  20. Schreiber LR, Gregory GF, Krause CR & Ichida JM (1988) Production, partial purification and antimicrobial activity of a novel antibiotic produced by a Bacillus subtilis from Ulmus americana. Can. J. Bot. 66: 2338–2346Google Scholar
  21. Thurston KC, Spencer SJ & Arditti W (1979) Phytotoxicity of fungicides and bacteriocides in orchid culture media. Amer. J. Bot. 66: 825–835Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • S. Hussain
    • 1
  • S. D. Lane
    • 1
  • D. N. Price
    • 1
  1. 1.Plant Biotechnology Unit, Department of Biological SciencesUniversity of PlymouthPlymouthUK

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