Plant Cell, Tissue and Organ Culture

, Volume 36, Issue 1, pp 45–51 | Cite as

A preliminary evaluation of the use of microbial culture filtrates for the control of contaminants in plant tissue culture systems

  • S. Hussain
  • S. D. Lane
  • D. N. Price


The culture extracts of 21 micro-organisms were screened in vitro for anti-microbial activity against a range of test contaminants isolated from plant tissue cultures. Extracts produced by Trichoderma viride Pers. (strain A) and Bacillus subtilis Ehrenberg (strain 2) had the highest antifungal activity and extracts from Pseudomonas fluorescens Migula (strain X) had the highest antibacterial activity. When extracts of these organisms were applied to Nicotiana tabacum L. cultures, they reduced the growth of accidental contaminants. In the case of B. subtilis and P. fluorescens strains, equivalent extracts did not reduce fresh weight yields of uncontaminated N. tabacum cultures over four growth passages.

Key words

callus contamination control 


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • S. Hussain
    • 1
  • S. D. Lane
    • 1
  • D. N. Price
    • 1
  1. 1.Plant Biotechnology Unit, Department of Biological SciencesUniversity of PlymouthPlymouthUK

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