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Plant Cell, Tissue and Organ Culture

, Volume 36, Issue 1, pp 15–19 | Cite as

Adventitious shoot regeneration from cultured petal explants of carnation

  • Masaru Nakano
  • Yoichiro Hoshino
  • Masahiro Mii
Article

Abstract

Adventitious shoot regeneration was compared among leaf, stem and petal explants of carnation (Dianthus caryophyllus L.) cv. Scania on MS medium containing different concentrations of 6-benzyladenine (BA) and α-naphthaleneacetic acid (NAA). High frequency regeneration was obtained only from petal explants on the media containing 5 to 10 μM BA with or without 5 μM NAA. Among the cytokinins tested, N-2-chloro-4-pyridyl-N′-phenylurea and N-1,2,3-thiadiazol-5-yl-N′-N′-phenylurea were more effective than BA, kinetin, N6-2-isopentenyl adenine and zeatin on regeneration from petal explants. Although, high frequency shoot regeneration was obtained from all petal explants harvested from various developmental stages of buds, a significant decrease in regeneration capacity was observed in the explants obtained from fully-opened flowers. High frequency shoot regeneration was also obtained from the petal explants of cvs. Coral. Lena, Nora and White Sim, and an interspecific cultivar Eolo using the method developed in this study.

Key words

cytokinins cultivars Dianthus caryophyllus organ formation petal culture 

Abbreviations

NAA

α-naphthaleneacetic acid

BA

6-benzyladenine

GA3

gibberellic acid

2iP

N6-2-isopentenyl adenine

KT-30

N-2-chloro-4-pyridyl-N′-phenylurea (also called 4PU)

TDZ

N-1,2,3-thiadiazol-5-yl-N′-phenylurea (also called thidiazuron)

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Masaru Nakano
    • 1
  • Yoichiro Hoshino
    • 1
  • Masahiro Mii
    • 1
  1. 1.Laboratory of Plant Cell Technology, Faculty of HorticultureChiba UniversityMatsudo, ChibaJapan

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