Photosynthesis Research

, Volume 26, Issue 2, pp 119–125

Consequences of the iron-dependent formation of ferredoxin and flavodoxin on photosynthesis and nitrogen fixation on Anabaena strains

  • Gerhard Sandmann
  • M. Luisa Peleato
  • Maria F. Fillat
  • M. Carmen Lázaro
  • Carlos Gómez-Moreno
Regular Papers

DOI: 10.1007/BF00047083

Cite this article as:
Sandmann, G., Peleato, M.L., Fillat, M.F. et al. Photosynth Res (1990) 26: 119. doi:10.1007/BF00047083

Abstract

Iron-dependent formation of ferredoxin and flavodoxin was determined in Anabaena ATCC 29413 and ATCC 29211 by a FPLC procedure. In the first species ferredoxin is replaced by flavodoxin at low iron levels in the vegetative cells only. In the heterocysts from Anabaena ATCC 29151, however, flavodoxin is constitutively formed regardless of the iron supply.

Replacement of ferredoxin by flavodoxin had no effect on photosynthetic electron transport, whereas nitrogen fixation was decreased under low iron conditions. As ferredoxin and flavodoxin exhibited the same Km values as electron donors to nitrogenase, an iron-limited synthesis of active nitrogenase was assumed as the reason for inhibited nitrogen fixation. Anabaena ATCC 29211 generally lacks the potential to synthesize flavodoxin. Under iron-starvation conditions, ferredoxin synthesis is limited, with a negative effect on photosynthetic oxygen evolution.

Key words

Anabaena ferredoxin flavodoxin FPLC determination of ferredoxin and flavodoxin Iron deficiency Nitrogen fixation Photosynthesis strain differences in gene expression 

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Gerhard Sandmann
    • 1
  • M. Luisa Peleato
    • 2
  • Maria F. Fillat
    • 2
  • M. Carmen Lázaro
    • 2
  • Carlos Gómez-Moreno
    • 2
  1. 1.Lehrstuhl für Physiologie und Biochemie der PflanzenUniversität KonstanzW. Germany
  2. 2.Departamento de Bioquímica y Biología Molecular y Celular, Facultad de CienciasUniversidad de ZaragozaSpain
  3. 3.Dr M.L. Peleato. Departamento de Bioquímica, Facultad de CienciasUniversidad de ZaragozaZaragozaSpain

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