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Plant Cell, Tissue and Organ Culture

, Volume 9, Issue 1, pp 19–27 | Cite as

Inexpensive precision temperature control for microculture

  • L. Art Spomer
  • Mary Ann Lila Smith
Article
  • 55 Downloads

Abstract

The design and construction of an inexpensive precision temperature control system used successfully for microculture applications is described. It is easily assembled from a homemade, precise, solid-state, proportional thermoregulator, resistive heating element, and insulated enclosure. The basic system easily adapts and can be customized for a variety of microculture applications with changes in enclosure dimensions or design. Control temperature accuracy, range and span can be varied by changing the components and design of the thermoregulator and enclosure. A series of such systems placed in a refrigerated or air-conditioned area accomodates multiple, parallel temperature treatments for experiments.

Key words

cell culture growth chamber incubator proportional thermoregulator tissue culture 

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References

  1. 1.
    Anonymous (1974) Thermistor Manual. Framingham, MA 01701: Fenwal Electronics, 33pGoogle Scholar
  2. 2.
    Batzer H (1979) Ecotoxiological aspects of engineering materials and in particular plastics. Chemosphere 11 & 12:877–885Google Scholar
  3. 3.
    Brown JA (1971) Temperature Measurement and Control. Boonton, NJ: RFL Industries, pp 7–14Google Scholar
  4. 4.
    Hammer PA, Langhans RW (1972) Experimental design consideration for growth chamber studies. HortScience 7:481–483Google Scholar
  5. 5.
    Hartman CL, McCoy TJ, Knous TR (1984) Selection of alfalfa (Medicago sativa) cell lines and regeneration of plants resistant to the toxin(s) produced by Fusarium oxysporum f. sp. medicagins. Plant Sci Letters 34:183–194Google Scholar
  6. 6.
    Holliday MJ, Klarman WL (1979) Expression of disease reaction types in soybean callus from resistant and susceptible plants. Phytopath 69:576–578Google Scholar
  7. 7.
    Howell EK (1969) Practical considerations of solid state electric heating controls-what, when, and how. IEEE Conf Rec 1969 Ind & Comm Power Systems and Elect Space Heating and Air Condit Joint Tech Conf:99–106Google Scholar
  8. 8.
    Hughes KE (1981) In vitro ecology: Exogenous factors affecting growth and morphogenesis in plant culture systems. Environ Expt Bot 21:281–288Google Scholar
  9. 9.
    Langhans RW (ed) (1978) A growth Chamber Manual: Environmental Control for Plants. Ithaca, NY 14853: Cornel Univ Press, 275 pGoogle Scholar
  10. 10.
    Malmstadt HV, Enke CG, Crouch SR (1973) Control of Electrical Quantities in Instrumentation. Menlo Park, CA: Benjamin, pp 167–173Google Scholar
  11. 11.
    McDonnell RE, Conger BV (1984) Callus induction and plantlet formation from mature embryo explants of Kentucky bluegrass. Crop Sci 24:573–578Google Scholar
  12. 12.
    Mulbach HP (1980) Different regeneration potentials of mesophyll protoplasts from cultivated and a wild species of tomato. Planta 148:89–96Google Scholar
  13. 13.
    Nickell L (1954) Nutritional aspects of virus-tumor growth. Brookhaven Symp Biol 6:174–186Google Scholar
  14. 14.
    Patrick OG (1966) Creative electronics Fabrication. Holt, Rinehart, and Winston, Inc. NY. 258 p.Google Scholar
  15. 15.
    Shepard J, Uyemoto J (1976) Influence of elevated temperatures on the isolation and proliferation of mesophyll protoplasts from PVX- and PVY-infected tobacco tissue. Virology 70:558–560Google Scholar
  16. 16.
    Spomer LA (1972) A simple, inexpensive water bath for precise temperature control. Agron J 64(6):837–838Google Scholar
  17. 17.
    Spomer LA (1982) A simple, inexpensive proportional water-bath temperature controller. J Phys E Sci Instrum 15:1163–1164Google Scholar
  18. 18.
    Tibbitts TW, McFarlane JC, Krizek DT, Berry WL, Hammer PA, Hodgson RH, Langhans RW (1977) Contaminants in plant growth chambers. HortScience 12:310–311Google Scholar
  19. 19.
    Unwin DM (1980) Microclimate Measurements for Ecologists. Academic Press, NY, pp 83–93Google Scholar
  20. 20.
    Widholm JM (1977) Relation between auxin autotrophy and tryptophan accumulation in cultured plant cells. Planta 134:103–108Google Scholar
  21. 21.
    Zapata J, Evans P, Power J, Cocking EC (1977) The effect of temperature on the division of leaf protoplasts of Lycopersicon esculentum and Lycopersicon peruvianum. Plant Sic Lett 8:119–124Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • L. Art Spomer
    • 1
  • Mary Ann Lila Smith
    • 1
  1. 1.Department of HorticultureUniversity of IllinoisUrbanaUSA

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