Development of techniques to maintain viable rotifer clones in a frozen state would preserve the genotype and reduce routine maintenance for those clones not being actively studied. To this end we have frozen Brachionus plicatilis in dimethyl sulfoxide at concentrations ranging from 6% to 18%. Survival rates decreased as the endpoint temperature was reduced from −20 °C to −45 °C, but did not decrease when the temperature was further reduced to −196 °C (liquid nitrogen). Only 2% of the individuals survived freezing in liquid nitrogen.
Keywordsrotifers cryopreservation Brachionus plicatilis
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- Ashwood-Smith, M. J. & Farrant, J., Eds., 1980. Low Temperature Preservation in Biology and Medicine. Pitman Medical Ltd., Kent. 323 pp.Google Scholar
- King, C. E., 1982. The evolution of lifespan. In: Dingle, H. & Hegmann, J. P. (Eds.). Evolution and Genetics of Life Histories. Springer-Verlag, New York: 121–138.Google Scholar
- Koehler, J. K., 1967. Studies on the survival of the rotifer Philodina after freezing and thawing. Cryobiology 3: 392–399.Google Scholar