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Plant Cell, Tissue and Organ Culture

, Volume 44, Issue 1, pp 83–86 | Cite as

In vitro regeneration of silktree (Albizzia julibrissin) from excised roots

  • D. Sankhla
  • Tim D. Davis
  • N. Sankhla
Research Note

Abstract

Root segments (1 cm long) were excised from 15–20 day old seedlings of silktree (Albizzia julibrissin) grown on B5 medium. About 50% of the control (no growth regulators added) root explants formed shoot buds within 15 days after placement on the culture medium. After 30 days, there were about 4 shoots per control explant. Addition of low levels of various auxins (0.5 μM) did not influence the formation of shoot buds from the explants. Higher concentrations (5μM), however, decreased shoot regeneration. Kinetin and 2iP did not influence shoot regeneration at the concentrations tested (1 & 10 μM). Addition of benzyladenine, Zeatin, or thidiazuron to the culture medium increased both the percentage of explants that formed shoots and the number of shoots per explant. Thidiazuron was highly effective in stimulating shoot formation at low concentrations (<1 μM). At 0.05 μM thidiazuron, 95% of the explants produced shoots and about 10 shoots were formed per explant. Compared to TDZ, higher concentrations (10 μM) of benzyladenine and Zeatin were required to enhance shoot formation. Upon excision and transfer to B5 medium, regenerated shoots developed into normal rooted plantlets.

Key words

adventitious shoot formation Albizzia julibrissin auxins cytokinins micropropagation silktree thidiazuron 

Abbreviations

BA

Benzyladenine

IAA

Indoleacetic acid

IBA

Indolebutyric acid

NAA

Naphthaleneacetic acid

TDZ

Thidiazuron

2ip

Isopentenyladenine

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • D. Sankhla
    • 1
  • Tim D. Davis
    • 1
  • N. Sankhla
    • 1
  1. 1.Texas Agricultural Experiment StationTexas A & M University Research and Extension CenterDallasUSA

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