Plant Molecular Biology

, Volume 25, Issue 3, pp 479–492 | Cite as

Expression of sunflower low-molecular-weight heat-shock proteins during embryogenesis and persistence after germination: localization and possible functional implications

  • María A. Coca
  • Concepción Almoguera
  • Juan Jordano
Research Articles


We isolated and sequenced Ha hsp 17.9, a DNA complementary (cDNA) of dry-seed stored mRNA that encodes a low-molecular-weight heat-shock protein (LMW HSP). Sequence analysis identified Ha hsp17.9, and the previously reported Ha hsp17.6, as cDNAs encoding proteins (HSP17.6 and HSP17.9) which belong to different families of cytoplasmic LMW HSPs. Using specific antibodies we observed differential expression of both proteins during zygotic embryogenesis under controlled environment, and a remarkable persistence of these LMW HSPs during germination. Immuno-blot analysis of HSP17.9 proteins in two-dimensional gels revealed that the polypeptides expressed in embryos were indistinguishable from LMW HSPs expressed in vegetative tissues in response to water deficit; but they appeared different from homologeous proteins expressed in response to thermal-stress. Tissue-print immunolocalization experiments showed that HSP17.9 and HSP17.6 were homogeneously distributed in every tissue of desiccation-tolerant dry seeds and young seedlings under non-stress conditions. These results demonstrate developmental regulation of specific, cytoplasmic, plant LMW HSPs, suggesting also their involvement in water-stress tolerance.

Key words

sunflower zygotic embryogenesis early germination cytoplasmic low-molecular weight heat-shock proteins desiccation tolerance heat-shock proteins 


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  1. 1.
    Lindquist S, Craig EA: The heat-shock proteins. Annu Rev Genet 22: 631–677 (1988).Google Scholar
  2. 2.
    Vierling E: The roles of heat shock proteins in plants. Annu Rev Plant Physiol Plant Mol Biol 42: 579–620 (1991).Google Scholar
  3. 3.
    Heikkila JJ: Heat shock gene expression and development. II. An overview of mammalian and avian developmental systems. Devel Genet 14: 87–91 (1993).Google Scholar
  4. 4.
    Winter J, Sinibaldi R: The expression of heat shock protein and cognate genes during plant development. In: Hightower L, Nover L (eds) Heat Shock and Development, pp. 85–105. Springer-Verlag, Berlin (1991).Google Scholar
  5. 5.
    Heikkila JJ: Heat shock gene expression and development. I. An overview of fungal, plant and poikilothermic animal developmental systems. Devel Genet 14: 1–5 (1993).Google Scholar
  6. 6.
    Bouchard RA: Characterization of expressed meiotic prophase repeat transcript clones ofLilium: meiosis-specific expression, relatedness and affinities to small heat shock protein genes. Genome 33: 68–79 (1990).Google Scholar
  7. 7.
    Atkinson BG, Raizada M, Bouchard RA, Frappier JRH, Walden DB: The independent stage-specific expression of the 18-kDa heat shock protein genes during micro-sporogenesis inZea mays L. Devel Genet 14: 15–26 (1993).Google Scholar
  8. 8.
    Kurtz S, Rossi J, Petko L, Lindquist S: An ancient developmental induction: heat shock proteins induced in sporulation and oogenesis. Science 231: 1154–1157 (1986).Google Scholar
  9. 9.
    Domoney C, Ellis N, Turner L, Casey R: A developmentally regulated early-embryogenis protein in pea (Pisum sativum L.) is related to the heat-shock protein (HSP70) gene family. Planta 184: 350–355 (1991).Google Scholar
  10. 10.
    Almoguera C, Jordano J. Developmental and environmental concurrent expression of sunflower dry-seedstored low-molecular-weight heat shock protein and lea mRNAs. Plant Mol Biol 19: 781–792 (1992).Google Scholar
  11. 11.
    Duck N, McCormick S, Winter J: Heat shock cognate gene expression in vegetative and reproductive organs ofLycopersicon esculentum. Proc Natl Acad Sci USA 86: 3674–3678 (1989).Google Scholar
  12. 12.
    Pitto J, Lo Schiavo F, Guiliano G, Terzi M: Analysis of the heat-shock protein pattern during somatic embryogenesis of carrot. Plant Mol Biol 2: 231–237 (1983).Google Scholar
  13. 13.
    Györgyey J, Gartner A, Németh K, Magyar Z, Hirt H, Herberle-Bors E, Dudits D: Alfalfa heat shock genes are differentially expressed during somatic embryogenesis. Plant Mol Biol 16: 999–1007 (1991).Google Scholar
  14. 14.
    DeRocher AE, Helm KE, Lauzon LM, Vierling E: Expression of a conserved family of cytoplasmic low molecular weight heat shock proteins during heat stress and recovery. Plant Physiol 96: 1038–1047 (1991).Google Scholar
  15. 15.
    Hernandez LD, Vierling E: Expression of low molecular weight heat-shock proteins under field conditions. Plant Physiol 101: 1209–1216 (1993).Google Scholar
  16. 16.
    Howarth C: Heat shock proteins inSorghum bicolor and Pennisetum americanum. II. Stored RNA in sorghum seed and its relationship to heat shock protein synthesis during germination. Plant Cell Envir 13: 57–64 (1990).Google Scholar
  17. 17.
    Helm KE, Abernethy RH: Heat shock proteins and their mRNAs in dry and early imbibing embryos of wheat. Plant Physiol 93: 1626–1633 (1990).Google Scholar
  18. 18.
    Vierling E, Sun A: Developmental expression of heat shock proteins in higher plants. In: Cherry J (ed) Environmental Stress in Plants. Biochemical and Physiological Mechanisms Associated with Environmental Stress Tolerance in Plants, pp. 343–354. Springer-Verlag, New York (1987).Google Scholar
  19. 19.
    Almoguera C, Coca MA, Jordano J: Tissue-specific expression of sunflower heat shock proteins in response to water stress. Plant J 4: 947–958 (1993).Google Scholar
  20. 20.
    DeRocher AE, Vierling E: Developmental control of small heat shock protein expression during pea seed maturation. Plant J 4: 93–102 (1994).Google Scholar
  21. 21.
    Bewley JD: Physiological aspects of dessication tolerance. Annu Rev Plant Physiol 30: 195–238 (1979).Google Scholar
  22. 22.
    Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989).Google Scholar
  23. 23.
    Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA. Selection of AUG initiation codon differs in plants and animals. EMBO J 6: 43–48 (1987).Google Scholar
  24. 24.
    Raschke E, Baumann G, Schoeffl F: Nucleotide sequence analysis of soybean small heat shock protein genes belonging to two different multigene families. J Mol Biol 199: 549–557 (1988).Google Scholar
  25. 25.
    Helm KE, Vierling E: AnArabidopsis cDNA clone encoding a low molecular heat shock protein. Nucl Acids Res 17: 7995 (1989).Google Scholar
  26. 26.
    Allen RD, Nessler CL, Thomas TL: Developmental expression of sunflower 11S storage protein genes. Plant Mol Biol 5: 165–173 (1985).Google Scholar
  27. 27.
    Gallie DR: Post-transcriptional regulation of gene expression in plants. Annu Rev Plant Physiol Plant Mol Biol 44: 77–105 (1993).Google Scholar
  28. 28.
    Robertson JA, ChapmanJr GW, WilsonJr RL: Relation of days after flowering to chemical composition and physiological maturity of sunflower seed. J Am Oil Chem Soc 55: 266–269 (1978).Google Scholar
  29. 29.
    Kruse E, Liu Z, Kloppstech K: Expression of heat shock proteins during development of barley. Plant Mol Biol 23: 111–122 (1993).Google Scholar
  30. 30.
    Wang C, Lin BL: The disappearance of an hsc70 species in mung bean seed during germination: purification and characterization of the protein. Plant Mol Biol 21: 317–329 (1993).Google Scholar
  31. 31.
    Dure LIII: The lea proteins of higher plants. In: Verma DPS (ed) Control of Plant Gene Expression, pp. 325–336. CRC Press, Boca Raton, FL (1993).Google Scholar
  32. 32.
    Ried JL, Walker-Simons MK. Group 3 late embryogenesis abundant proteins in desiccation-tolerant seedlings of wheat (Triticum aestivum L.). Plant Physiol 102: 125–131 (1993).Google Scholar
  33. 33.
    Schneider K, Wells B, Schmelzer E, Salamini F, Bartels D. Desiccation leads to the rapid accumulation of both cytosolic and chloroplastic proteins in the resurrection plantCraterostigma plantagineum Hochst. Planta 189: 120–131 (1993).Google Scholar
  34. 34.
    Roberts JK, DeSimone NA, Lingle WL, Dure LIII: Cellular concentrations and uniformity of cell-type accumulation of two lea proteins in cotton embryos. Plant Cell 5: 769–780 (1993).Google Scholar
  35. 35.
    Chirico WJ, Waters MG, Blobel G: 70K heat shock related proteins stimulate protein translocation into microsomes. Nature 332: 805–810 (1988).Google Scholar
  36. 36.
    Jakob U, Gaestel M, Engel K, Buchner J: Small heat shock proteins are molecular chaperones. J Biol Chem 268: 1517–1520 (1993).Google Scholar
  37. 37.
    Lavoie JN, Gingrasbreton G, Tanguay RM, Landry I: Induction of Chinese hamster HSP27 gene expression in mouse cells confers resistance to heat shock. HSP27 stabilization of the microfilament organization. J Biol Chem 268: 3420–3429 (1993).Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • María A. Coca
    • 1
  • Concepción Almoguera
    • 1
  • Juan Jordano
    • 1
  1. 1.Instituto de Recursos Naturales y AgrobiologíaC.S.I.C.SevillaSpain

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