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Plant Cell, Tissue and Organ Culture

, Volume 13, Issue 3, pp 167–177 | Cite as

Growth and activities of enzymes of primary metabolism in batch cultures of Catharanthus roseus cell suspension under different pCO2 conditions

  • J. P. Ducos
  • G. Feron
  • A. Pareilleux
General Papers

Abstract

In vitro enzyme activities of glycolysis, pentose-phosphate pathway and dark CO2 fixation were assayed in batch cultures of heterotrophic Catharanthus roseus cells under various gassing rates and partial pressures of carbon dioxide. Detrimental effects of low pCO2 culture conditions on the growth characteristics could be linked to marked changes in levels of enzymes of primary metabolism during growth. The enzyme levels observed during the early stages of growth were found to be more stable when a constant pCO2 (20 mbar) was maintained and enabled exponential growth to be reached more rapidly.

The importance of carbon dioxide as a “conditioning factor” of the culture medium is discussed.

Key words

Catharanthus roseus cell suspension culture enzyme activities carbon dioxide partial pressure 

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References

  1. 1.
    Bown AW (1985) CO2 and intracellular pH. Plant Cell and Environ 8: 459–465Google Scholar
  2. 2.
    Ducos JP, Pareilleux A (1986) Effect of aeration rate and influence of pCO2 in large-scale cultures of Catharanthus roseus cells. Appl Microbiol Biotechnol 25: 101–105Google Scholar
  3. 3.
    Fordyce AM, Moore HC, Pritchard GG (1982) Phosphofructokinase in Streptococcus lactis. In: Wood WA (ed) Methods in Enzym 90, pp 77–82 Academic Press, New YorkGoogle Scholar
  4. 4.
    Fowler MW, Clifton A (1974) Activites of carbohydrate metabolism in cells of Acer pseudoplatanus 8maintained in continuous chemostat culture. Eur J Biochem 45: 445–450Google Scholar
  5. 5.
    Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension culture of soybean root cells. Exp Cell Res 50: 151–158Google Scholar
  6. 6.
    Gathercole RWE, Mansfield KJ, Street HE (1976) Carbon dioxide as an essential requirement for cultured sycamore cells. Physiol Plant 37: 213–217Google Scholar
  7. 7.
    Hegarty PK, Smart NJ, Scragg AH, Fowler MW (1986) The aeration of Catharanthus roseus (L.) G. Don suspension cultures in air-lift bioreactors: the inhibitory effect at high aeration rates on culture growth. J Exp Bot 37: 1911–1922Google Scholar
  8. 8.
    Kato A, Shimizu Y, Nagai S (1975) Effect of initial kl.a on the growth of tobacco cells in batch culture. J Ferment Technol 53: 744–751Google Scholar
  9. 9.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265–275Google Scholar
  10. 10.
    Maurel B, Pareilleux A (1985) Effect of carbon dioxide on the growth of cell suspensions of Catharanthus roseus. Biotechnol Letters 7: 313–318Google Scholar
  11. 11.
    Maurel B, Pareilleux A (1986) Carbon dioxide fixation and growth of heterotrophic cell suspensions of Catharanthus roseus. J Plant Physiol 122: 347–355Google Scholar
  12. 12.
    Nesius KK, Fletcher JS (1973) Carbon dioxide and pH requirements of non-photosynthetic tissue culture cells. Physiol Plant 28: 259–263Google Scholar
  13. 13.
    Pareilleux A, Chaubet N (1981) Mass cultivation of Medicago sativa growing on lactose: kinetic aspects. J Appl Microbiol Biotechnol 11: 222–225Google Scholar
  14. 14.
    Schnabl H, Mayer I (1976) Dark fixation of CO2 by flowers of cut roses. Planta 1311: 51–55Google Scholar
  15. 15.
    Scott WA (1975) Glucose-6-phosphate dehydrogenase from Neurospora crassa. In: Wood WA (ed) Methods in Enzymology 41, pp 177–182. Academic Press, New YorkGoogle Scholar
  16. 16.
    Scott WA (1975) 6-phosphogluconate dehydrogenase from Neurospora crassa. In: Wood WA (ed) Methods in Enzymology 41, pp 227–231. Academic Press, New YorkGoogle Scholar
  17. 17.
    Smart NJ, Fowler MW (1981) Effect of aeration on large-scale cultures of plant cells. Biotechnol Letters 3: 171–176Google Scholar
  18. 18.
    Tanaka H (1982) Oxygen transfer in broths of plant cell at high density. Biotechnol Bioeng 24: 425–442Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • J. P. Ducos
    • 1
  • G. Feron
    • 1
  • A. Pareilleux
    • 1
  1. 1.Département de Génie Biochimique et Alimentaire, UA-CNRS-N°0 544Institut National des Sciences AppliquéesToulouse CédexFrance

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