Somatic embryogenesis in cultured mature zygotic embryos of Abies balsamea
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Embryo suspensor masses (ESMs) were induced by culture of isolated mature zygotic embryos of balsam fir [Abies balsamea (Mill.)] on media containing 10 μM cytokinin [6-(γ-γ-dimethylallylamino)purine (2iP), 6-benzyladenine (BA), or thidiazuron (TDZ)]. Once induced, ESMs proliferated on media containing 2iP, BA or TDZ (10 μM) or on 4.5 μM BA in combination with 10 μM naphthyl-1-acetic acid. When ESMs were transferred to media containing 5–80 μM abscisic acid, cotyledonary-stage embryos were formed. Embryos were readily germinated on medium lacking growth regulators.
Key wordsconifers cytokinins
N-phenyl-N′-1,2,3-thiadiazol-5-yl urea (thidiazuron)
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