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Plant Cell, Tissue and Organ Culture

, Volume 45, Issue 1, pp 79–85 | Cite as

Efficient propagation of protocorm-like bodies of Phalaenopsis in liquid medium

  • Yong Soo Park
  • Syuuichi Kakuta
  • Atsushi Kano
  • Mitsuyasu Okabe
Original Research Paper

Abstract

Propagation by multiplication of protocorm-like bodies (PLB) of Phalaenopsis in liquid culture was investigated. VW-liquid medium was the most suitable for PLB multiplication, which showed 30% PLB multiplication. A cotton plate was chosen as a reactor for PLB propagation. The PLB in VW-liquid medium multiplied 80% with non-absorbent cotton as a PLB support, as opposed to 70% in VW-solid medium; the PLB growth rate in that medium was much higher than that in the solid culture. When the cotton thickness was 6.0 mm that was corresponded to four layers of absorbent cotton and medium content containing the cotton was 7.2 ml g-1 cotton, the PLB multiplication ratio was 100%; the most effective for the propagation of PLB. This indicates that there is an optimum wet condition in the liquid culture for an efficient PLB propagation.

Key words

Cotton plate micropropagation Phalaenopsis protocorm-like body 

Abbreviations

BA

6-benzyladenine

NAA

α-naphthaleneacetic acid

MS

Murashige & Skoog basal medium

PLB

protocorm-like body

VW

Vacin & Went

TBR

turbine blade reactor

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Yong Soo Park
    • 1
  • Syuuichi Kakuta
    • 1
  • Atsushi Kano
    • 2
  • Mitsuyasu Okabe
    • 1
  1. 1.Biochemical Engineering Lab., Department of Applied Biological ChemistryShizuoka UniversityShizuokaJapan
  2. 2.Department of Biological ScienceShizuoka UniversityShizuokaJapan

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