Plant Cell, Tissue and Organ Culture

, Volume 18, Issue 3, pp 321–327 | Cite as

Peroxidase activity in calluses and cell suspension cultures of radishRaphanus sativus var. Cherry Bell

  • Oscar A. Moreno V.
  • Rafael Vazquez-Duhalt
Short Communication

Abstract

Calluses ofRaphanus sativus var. Cherry Bell were induced in a medium containing 2,4-dichlorophenoxyacetic acid and benzyladenine. The biomass and peroxidase activities were determined, both in agar cultures and cell suspension cultures. Different growth-regulator concentrations induced different responses measured as peroxidase activity in callus. The suspended-cell cultures showed the importance of selecting the cell line, in order to obtain an optimal response in extracellular peroxidase activity. The commercial production of this enzyme by utilizing plant cell tissue cultures is discussed.

Key words

Raphanus sativus peroxidase callus cell suspension extracellular enzyme 

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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • Oscar A. Moreno V.
    • 1
  • Rafael Vazquez-Duhalt
    • 1
  1. 1.Centro de Investigaciones Biológicas de Baja California SurLa PazMéxico

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