Plant Cell, Tissue and Organ Culture

, Volume 13, Issue 1, pp 61–76 | Cite as

Tissue culture and plant regeneration from sunflower (Helianthus annuus) and interspecific hybrids (H. tuberosus x H. annuus)

  • B. Witrzens
  • W. R. Scowcroft
  • R. W. Downes
  • P. J. Larkin


A method is described for the culture and regeneration of plants from callus of sunflower (Helianthus annuus) andH. annuus x H. tuberosus hybrids. Immature embryos proved to be the only explant which consistently gave regenerable cultures in all genotypes. The most responsive embryos were approximately 12 mm2 in area. Genotype had a significant effect on the capacity of cultures to regenerate. Some regeneration was also obtained from cultures of tuber tissue but only from one genotype,H. tuberosus x H. annuus cross 200. None of theH. annuus accessions gave regenerable callus from root tissue. Difficulties included the premature initiation of flowering of regenerating shoots and the frequent occurence of "vitreous" plantlets which could not be transplanted successfully to soil. Some amelioration of both these problems was achieved by replacing inorganic nitrogen partially with amino acids. More effective reduction of these difficulties was accomplished by the addition of 10, 30 and 100 μM phloridzin, esculin or naringin.

Key words

Helianthus annuus Helianthus tuberosus sunflower regeneration immature embryos phloridzin 



6-benzylaminopurine; zeatin, trans-6-(4-hydroxy-3-methyl-but-2-enyl) aminopurine; kinetin, 6-furfurylaminopurine


indole-acetic acid


naphthyl acetic acid


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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • B. Witrzens
    • 1
  • W. R. Scowcroft
    • 1
  • R. W. Downes
    • 1
  • P. J. Larkin
    • 1
  1. 1.Division of Plant IndustryCSIROCanberraAustralia

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