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Plant Molecular Biology

, Volume 23, Issue 6, pp 1273–1278 | Cite as

Isolation and characterization of a polygalacturonase gene highly expressed in Brassica napus pollen

  • Laurian S. Robert
  • Sharon Allard
  • Jean L. Gerster
  • Leslie Cass
  • John Simmonds
Short Communication

Abstract

A cDNA clone, Sta 44-4, corresponding to a mRNA highly expressed in Brassica napus cv. Westar stamens, was isolated by differential screening and characterized. Northern blot and in situ analyses demonstrated that Sta 44-4 is synthesized in pollen beginning at the late uninucleate stage and reaches a maximum in trinucleate microspores. Sta 44-4 displayed significant sequence similarity to known pollen polygalacturonase genes. The B. napus pollen polygalacturonase gene was shown to be part of a small gene family and to display some polymorphism among different cultivars.

Key words

Brassica napus pollen polygalacturonase 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Laurian S. Robert
    • 1
  • Sharon Allard
    • 1
  • Jean L. Gerster
    • 1
  • Leslie Cass
    • 1
  • John Simmonds
    • 1
  1. 1.Plant Research CentreCentral Experimental FarmOttawaCanada

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