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Plant Cell, Tissue and Organ Culture

, Volume 32, Issue 3, pp 289–292 | Cite as

Plant regeneration via somatic embryogenesis in ginger

  • A. Kackar
  • S. R. Bhat
  • K. P. S. Chandel
  • S. K. Malik
Research Articles

Abstract

Embryogenic callus cultures of ginger were induced from young leaf segments taken from in vitro shoot cultures. Among the four auxins tested in Murashige & Skoog medium, dicamba at 2.7 μM was most effective in inducing and maintaining embryogenic cultures. Efficient plant regeneration was achieved when embryogenic cultures were transferred to Murashige & Skoog medium containing 8.9 μM benzyladenine. Histological studies revealed various stages of somatic embryogenesis characteristic of the monocot system. The in vitro-raised plants have been established in soil.

Key words

callus culture histology young leaf Zingiber officinale 

Abbreviations

BA

benzyladenine

2,4-d

2,4-dichlorophenoxyacetic acid

IAA

indole-3-acetic acid

MS

Murashige and Skoog

NAA

naphthaleneacetic acid

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • A. Kackar
    • 1
  • S. R. Bhat
    • 1
  • K. P. S. Chandel
    • 1
  • S. K. Malik
    • 1
  1. 1.National Facility for Plant Tissue CultureNew DelhiIndia

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