Plant Molecular Biology

, Volume 31, Issue 3, pp 465–478 | Cite as

A wound-inducible gene fromSalix viminalis coding for a trypsin inhibitor

  • Peter Saarikoski
  • David Clapham
  • Sara von Arnold
Regular Article


A gene designatedswin1.1 has been isolated by screening aSalix viminalis genomic library with a heterologous probe,win3 fromPopulus. The region sequenced included the entire coding sequence for a protein with 199 amino acids plus the promoter and terminator. At the 5′ end of the coding region is a sequence that encodes a hydrophobic region of 25–30 amino acids, that could form a signal peptide. A putative TATAA box and polyadenylator sequence were identified. Introns were absent. The gene product showed similarities with serine protease inhibitors from the Kunitz family and especially withwin3 from wounded leaves ofPopulus. Southern blot analysis indicated thatswin1.1 is a member of a clustered gene family,swin1. An oligonucleotide corresponding to the putative hypervariable region to-wards the carboxyl end when used as a probe in Southern hybridization showed high specificity forswin1.1. Expression of theswin1.1 gene was enhanced in wounded leaves. Theswin1.1 coding region without the signal sequence was highly expressed inEscherichia coli and the protein showed inhibitory activity against trypsin but at most slight activity against the other proteases tested. A systemically induced protein, SVTI, with inhibitor activity against trypsin, was isolated fromSalix leaves by affinity chromatography on a column of trypsin-Sepharose 4B and N-terminal sequenced. It corresponded with the translatedswin1.1 gene at 16 of the 19 amino acid sites, suggesting that SVTI is encoded by another member of theswin1 gene family.

Key words

hypervariable region Kunitz family Salix viminalis serine protease inhibitor wound-induced 


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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Peter Saarikoski
    • 1
  • David Clapham
    • 1
  • Sara von Arnold
    • 1
  1. 1.Department of Forest Genetics, The Genetic CenterSwedish University of Agricultural SciencesUppsalaSweden

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