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Plant Cell, Tissue and Organ Culture

, Volume 25, Issue 2, pp 99–105 | Cite as

Adventitious shoot regeneration from in vitro leaves of several pear cultivars (Pyrus communis L.)

  • C. Leblay
  • E. Chevreau
  • L. M. Raboin
Article
  • 297 Downloads

Abstract

An efficient adventitious shoot regeneration system was developed for pear (Pyrus communis L.), using leaves from in vitro proliferating shoots. Under optimal conditions, bud regeneration frequencies of ‘Comice’, ‘Passe-Crassane’, ‘Williams’ and ‘Conference’ ranged from 60% to 97%, with the mean number of shoots per regenerating leaf ranging from 3.2 to 6.6. Despite the great variability in responses of the different cultivars, in general an initial dark exposure of at least 20 days was required. Ammonium and total nitrogen proved to play an essential role: intermediate NH4+ concentrations were suitable for regeneration. The balance between NH4+ and NO3- also influenced regeneration; optimal regeneration occured on media with a 1:3 NH4+/NO3- ratio. TDZ at 1 μM was less efficient than higher concentrations, whatever the NAA level. Finally, length and growth regulator composition of the two phases (induction and expression) influenced the regeneration rate of ‘Conference’.

Key words

adventitious bud regeneration in vitro culture Pyrus communis L. 

Abbreviations

BA

6-benzyladenine

EDFS

ethylenediamine-tetraacetic acid ferric-sodium salt

IBA

4-indole-3yl-butyric acid

NAA

α-naphthaleneacetic acid

TDZ

thidiazuron (N-phenyl-N′-1,2,3-thidiazol-5-ylurea)

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • C. Leblay
    • 1
  • E. Chevreau
    • 1
  • L. M. Raboin
    • 1
  1. 1.Station d'Amélioration des Espèces Fruitières et OrnementalesINRAAngersFrance

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