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Plant Molecular Biology

, Volume 32, Issue 6, pp 1197–1203 | Cite as

Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppression

  • Paul D. Cluster
  • Michael O'Dell
  • Michael Metzlaff
  • Richard B. Flavell
Short Communication

Abstract

Analysis of Agrobacterium-transferred DNA (T-DNA) revealed strong correlations between transgene structures and floral pigmentation patterns from chalcone synthase (chs) co-suppression among 47 Petunia transformants. Presented here are the full details of T-DNA structural organization in that population. Sixteen transformants (34%) carried one T-DNA copy while 31 (66%) carried 106 complete and partial T-DNA elements in 54 linkage groups. Thirty linkage groups contained multiple T-DNA copies; 15 of these contained only contiguously repeated copies, 8 contained only dispersed copies and 7 contained both. Right-border inverted repeats were three times more frequent than left-border inverted or direct repeats. Large fragments of binary-vector sequences were linked to the T-DNA in seven plants.

Key words

Agrobacterium-mediated transformation binary-verector transfer direct repeats inverted repeats repeat-induced gene silencing transgene inheritance 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Paul D. Cluster
    • 1
  • Michael O'Dell
    • 1
  • Michael Metzlaff
    • 1
  • Richard B. Flavell
    • 1
  1. 1.John Innes CentreNorwichUK

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