Plant Cell, Tissue and Organ Culture

, Volume 40, Issue 1, pp 33–35 | Cite as

Plant regeneration from shoot apex explants of foxtail millet

  • Pedro Osuna-Avila
  • Alejandro Nava-Cedillo
  • Alba E. Jofre-Garfias
  • José Luis Cabrera-Ponce
Original Research Papers
Received:
Accepted:

Abstract

Green and etiolated shoot apices of foxtail millet (Setaria italica L.) cv. Nese 2A were cultured on Murashige and Skoog medium with four concentrations of 2,4-dichlorophenoxyacetic acid or 2,4,5-trichlorophenoxyacetic acid. In all treatments, embryogenic calli capable of plant regeneration were induced after ten weeks in culture. Calli induced on 2 mg l-1 of 2,4-d from green apices gave a higher rate of plant regeneration in comparison with etiolated apices on the other treatments. Plant regeneration was obtained from one year-old cultures. Regenerated plants were successfully established in soil, reached maturity and produced seeds.

Key words

Setaria italica 

Abbreviations

2,4-d

2,4-dichlorophenoxyacetic acid

EC

embryogenic calli

NE

nonembryogenic calli

2,4,5-T

2,4,5-trichlorophenoxyacetic acid

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Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Pedro Osuna-Avila
    • 1
  • Alejandro Nava-Cedillo
    • 1
  • Alba E. Jofre-Garfias
    • 2
  • José Luis Cabrera-Ponce
    • 2
  1. 1.Central CamioneraCIGA ITA 20Aguascalientes, Ags.México
  2. 2.Depto. de Ingeniería Genética de PlantasCINVESTAV-IPNIrapuato, Gto.México

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