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Plant Molecular Biology

, Volume 31, Issue 6, pp 1227–1231 | Cite as

Ethylene receptor expression is regulated during fruit ripening, flower senescence and abscission

  • Sharon Payton
  • Rupert G. Fray
  • Stephen Brown
  • Don Grierson
Short Communication

Abstract

Using theArabidopsis ethylene receptorETR1 as a probe, we have isolated a tomato homologue (tETR) from a ripening cDNA library. The predicted amino acid sequence is 70% identical toETR1 and homologous to a variety of bacterial two component response regulators over the histidine kinase domain. Sequencing of four separate cDNAs indicates that tETR lacks the carboxyl terminal response domain and is identical to that encoded by the tomatoNever ripe gene. Ribonuclease protection showed tETR mRNA was undetectable in unripe fruit or pre-senescent flowers, increased in abundance during the early stages of ripening, flower senescence, and in abscission zones, and was greatly reduced in fruit of ripening mutants deficient in ethylene synthesis or response. These results suggest that changes in ethylene sensitivity are mediated by modulation of receptor levels during development.

Key words

abscission ethylene receptor ripening senescence tomato 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Sharon Payton
    • 1
  • Rupert G. Fray
    • 1
  • Stephen Brown
    • 1
  • Don Grierson
    • 1
  1. 1.BBSRC Research Group in Plant Gene Regulation, Department of Physiology and Environmental ScienceUniversity of NottinghamLoughboroughUK

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