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The influence of cytokinin pulse treatments on adventitious bud formation on vegetative buds of Picea abies


Resting vegetative buds of Picea abies collected from phytotron-grown rooted cuttings of 24-year-old trees or a 12-year-old hedge were tested for their capacity to form adventitious buds after various cytokinin treatments. The most effective method for obtaining a high yield of adventitious buds within 8 weeks was to pulse treat the buds in 250 μM BA for 3 h and then culture them on medium containing 5 μM each of BA and kinetin for 1 week. The developmental pattern for adventitious bud production, with the formation of 10 to 20 adventitious buds per bud, was similar for all tested genotypes, although the number of buds giving rise to adventitious buds varied significantly. The capability of some clones to form adventitious buds was correlated to endogenous cytokinin content. The clone which contained most endogenous cytokinin in its resting bud had the highest potential for adventitious bud formation.

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Von Arnold, S., Tillberg, E. The influence of cytokinin pulse treatments on adventitious bud formation on vegetative buds of Picea abies . Plant Cell Tiss Organ Cult 9, 253–261 (1987).

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  • Plant Physiology
  • High Potential
  • Kinetin
  • Developmental Pattern
  • Rooted Cutting