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Plant Cell, Tissue and Organ Culture

, Volume 9, Issue 3, pp 245–252 | Cite as

Callus induction and plant regeneration from shoot portions of mature embryos of high tannin sorghums

  • Tishu Cai
  • Barbara Daly
  • Larry Butler
Short Communications

Abstract

Callus and plant regenertion were induced from shoot portions of mature embryos (dry seeds) of five high tannin Sorghum bicolor (L.) Moench cultivars. The explants were cultured on Murashige and Skoog medium with altered concentrations of 5 salts, supplemented with 150 mg/L L-asparagine, 5mg/L 2,4-Dichlorophenoxyacetic acid and 0.05mg/L kinetin. Calli which were yellow and globular were formed with 70–90% frequencies. The subculture medium which gave best results was MS with 2mg/L 2,4-Dichlorophenoxyacetic acid and 0.5mg/L kinetin. Plants were regenerated on MS medium supplemented with 150mg/L L-asparagine and 0.2mg/L kinetin with regeneration frequencies of 11–48%.

Key words

cereal tissue culture plant regeneration Sorghum bicolor 

Abbreviations

2,4-D

dichlorophenoxyacetic acid

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Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • Tishu Cai
    • 1
  • Barbara Daly
    • 1
  • Larry Butler
    • 1
  1. 1.Department of BiochemistryPurdue UniversityWest LafayetteUSA

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