Plant Molecular Biology

, Volume 31, Issue 5, pp 1073–1077

Molecular cloning of a tomato leaf cDNA encoding an aspartic protease, a systemic wound response protein

  • Andreas Schaller
  • Clarence A. Ryan
Short Communication

Abstract

A full-length cDNA encoding an aspartic protease (LeAspP) has been cloned from a tomato leaf cDNA library. Using LeAspP cDNA as a probe in gel blots, LeAspP mRNA was shown to be systemically induced in tomato leaves by wounding. Application of methyl jasmonate to leaves of intact tomato plants, or supplying systemin to young tomato plants through their cut stems, induces synthesis of LeAspP mRNA. LeAspP message is regulated in tomato similar to several systemic wound response proteins (swrps) that are part of the defense response in tomato plants directed against herbivore attacks.

Key words

aspartic protease methyl jasmonate plant defense systemin tomato leaves 

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Andreas Schaller
    • 1
  • Clarence A. Ryan
    • 1
  1. 1.Institute of Biological ChemistryWashington State UniversityPullman
  2. 2.Institut für Pflanzenwissenschaften, ETH ZürichZürichSwitzerland

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