Plant Molecular Biology

, Volume 26, Issue 4, pp 1055–1064 | Cite as

The ocs element in the soybean GH2/4 promoter is activated by both active and inactive auxin and salicylic acid analogues

  • Tim Ulmasov
  • Gretchen Hagen
  • Tom Guilfoyle
Research Article

Abstract

The octopine synthase (ocs or ocs-like) element has been previously reported to be responsive to the plant hormones, auxin, salicylic acid, and methyl jasmonate. Using transient assays with carrot protoplasts, we have demonstrated that an ocs element from the soybean auxin-inducible GH2/4 promoter is not only activated by strong auxins (i.e, 2,4-dichlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid, α-naphthalene acetic acid) and salicylic acid, but also by weak auxin analogues (β-naphthalene acetic acid), inactive auxin analogs (i.e., 2,3-dichlorophenoxyacetic acid, 2,4,6-trichlorophenoxyacetic acid), and inactive salicylic acid analogs (3-hydroxybenzoic acid and 4-hydroxybenzoic acid). Our results indicate that the ocs element in the GH2/4 promoter is not selectively induced by plant hormones and might function similarly to tandem AP-1 sites in some animal glutathione S-transferase (GST) genes. The ocs element, like the AP-1 sites in animal GST promoters, may be induced not only by certain hormones but also by some non-hormonal stress-inducing or electrophilic agents.

Key words

soybean octopine synthase element plant hormones auxin salicylic acid 

Abbreviations

GST

glutathione S-transferase

MUG

4-methyl-umbelliferyl-glucuronide

GUS

β-glucuronidase

2,4-D

2,4-dichlorophenoxyacetic acid

2,3-D

2,3-dichlorophenoxyacetic acid

2,4,5-T

2,4,5-trichlorophenoxyacetic acid

2,4,6-T

2,4,6-trichlorophenoxyacetic acid

NAA

naphthalene acetic acid

SA

salicylic acid

SARE

putative salicylic acid-responsive element

BA

benzoic acid

UTR

untranslated region

nos

nopaline synthase

ocs

octopine synthase

mas

mannopine synthase

ocs

element-(−)46 CaMV 35S promoter-GUS reporter gene: the ocs element fused to a minimal −46 cauliflower mosaic virus 35S promoter fused to a GUS reporter gene with a 3′ nos untranslated region

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Tim Ulmasov
    • 1
  • Gretchen Hagen
    • 1
  • Tom Guilfoyle
    • 1
  1. 1.Department of BiochemistryUniversity of MissouriColumbiaUSA

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