Plant Molecular Biology

, Volume 18, Issue 3, pp 623–627 | Cite as

Isolation and characterization of a plant cDNA showing homology to animal glutathione peroxidases

  • M. C. Criqui
  • E. Jamet
  • Y. Parmentier
  • J. Marbach
  • A. Durr
  • J. Fleck
Update Section Short Communication


A cDNA library from freshly isolated protoplasts was differentially screened using cDNAs from mesophyll cells, stressed leaf strips and cell suspension cultures. One of the selected clones, 6P229, turned out to encode a putative polypeptide showing homology to the btuE periplasmic protein of Escherichia coli and to animal selenium-dependent glutathione peroxidases. A major difference was that the putative selenocysteine in the active site was not encoded by the termination codon TGA. The 6P229 gene was found to be expressed in germinating seeds, in apex and in flowers, as well as in stressed tissues. This pattern of expression would be consistent with a key role in cellular metabolism such as defense against oxidative stresses.

Key words

btuE glutathione peroxidase Nicotiana sylvestris protoplast stress 


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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • M. C. Criqui
    • 1
  • E. Jamet
    • 1
  • Y. Parmentier
    • 1
  • J. Marbach
    • 1
  • A. Durr
    • 1
  • J. Fleck
    • 1
  1. 1.Institut de Biologie Moléculaire des Plantes du C.N.R.S.StrasbourgFrance

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