Plant Molecular Biology

, Volume 20, Issue 3, pp 555–558

Expression of desiccation-related proteins from the resurrection plant Craterostigma plantagineum in transgenic tobacco

  • Gabriel Iturriaga
  • Katharina Schneider
  • Francesco Salamini
  • Dorothea Bartels
Update Section Short Communication

Abstract

Three cDNAs encoding desiccation-induced proteins from the resurrection plant Craterostigma plantagineum were each ligated to a triplicated CaMV 35S promoter and a nopaline synthase 3′-flanking region in an Agrobacterium vector and introduced into tobacco. Transgenic plants expressed the encoded Craterostigma proteins at high levels. This did not lead to changes in the phenotype, in the growth habit or in basic photosynthetic parameters. In tobacco, one protein was targeted to the chloroplast stroma which is its normal location in Craterostigma. These desiccation-related proteins are not sufficient per se to increase drought tolerance as measured by ion-leakage tests.

Key words

Chloroplast targeting desiccation tolerance drought-related proteins resurrection plant (Craterostigmatransgenic tobacco 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Gabriel Iturriaga
    • 1
  • Katharina Schneider
    • 1
  • Francesco Salamini
    • 1
  • Dorothea Bartels
    • 1
  1. 1.Max-Planck-Institut für ZüchtungsforschungKöln 30Germany
  2. 2.Centro de Investigacion sobre Ingenieria Genetica y BiotechnologiaUniversidad Nacional Autonoma de MexicoCuernavacaMexico

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