Plant Molecular Biology

, Volume 20, Issue 3, pp 555–558

Expression of desiccation-related proteins from the resurrection plant Craterostigma plantagineum in transgenic tobacco

  • Gabriel Iturriaga
  • Katharina Schneider
  • Francesco Salamini
  • Dorothea Bartels
Update Section Short Communication


Three cDNAs encoding desiccation-induced proteins from the resurrection plant Craterostigma plantagineum were each ligated to a triplicated CaMV 35S promoter and a nopaline synthase 3′-flanking region in an Agrobacterium vector and introduced into tobacco. Transgenic plants expressed the encoded Craterostigma proteins at high levels. This did not lead to changes in the phenotype, in the growth habit or in basic photosynthetic parameters. In tobacco, one protein was targeted to the chloroplast stroma which is its normal location in Craterostigma. These desiccation-related proteins are not sufficient per se to increase drought tolerance as measured by ion-leakage tests.

Key words

Chloroplast targeting desiccation tolerance drought-related proteins resurrection plant (Craterostigmatransgenic tobacco 


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  1. 1.
    Bartels D, Schneider K, Terstappen G, Piatkowski D, Salamini F: Molecular cloning of abscisic acid-modulated genes which are induced during desiccation of the resurrection plant Craterostigma plantagineum. Planta 181: 27–34 (1990).Google Scholar
  2. 2.
    Bevan MW: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).Google Scholar
  3. 3.
    Blum A, Ebercon A: Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Sci 21: 43–47 (1991).Google Scholar
  4. 4.
    Guilley H, Dudley K, Jonard G, Richards K, Hirth L: Transcription of cauliflower mosaic virus DNA: Detection of promoter sequences, and characterization of transcripts. Cell 21: 285–294 (1982).Google Scholar
  5. 5.
    Mundy J, Chua N-H: Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J 7: 2279–2286 (1988).Google Scholar
  6. 6.
    Piatkowski D, Schneider K, Salamini F, Bartels D: Characterization of five abscisic acid-responsive cDNA clones isolated from the desiccation-tolerant plant Craterostigma plantagineum and their relationship to other water-stress genes. Plant Physiol 94: 1682–1688 (1990).Google Scholar
  7. 7.
    Skriver K, Mundy J: Gene expression in response to abscisic acid and osmotic stress. Plant Cell 2: 503–512 (1990).Google Scholar
  8. 8.
    Sullivan CY: Mechanisms of heat and drought resistance in grain sorghum and methods of measurement. In: Sorghum in the Seventies, pp. 247–264. Oxford University Press, Oxford/IBH Publishing Co, New Delhi (1972).Google Scholar
  9. 9.
    Yancey PH, Clark ME, Hand SC, Bowlus RD, Somero GN: Living with water stress: Evolution of osmolyte systems. Science 217: 1214–1222 (1982).Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Gabriel Iturriaga
    • 1
  • Katharina Schneider
    • 1
  • Francesco Salamini
    • 1
  • Dorothea Bartels
    • 1
  1. 1.Max-Planck-Institut für ZüchtungsforschungKöln 30Germany
  2. 2.Centro de Investigacion sobre Ingenieria Genetica y BiotechnologiaUniversidad Nacional Autonoma de MexicoCuernavacaMexico

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